In vitro analysis of versican in limb chondrogenesis utilizing the hdf transgenic mouse / by Danielle Marie Gillotte.
| Author/creator | Gillotte, Danielle Marie author. |
| Other author | Capehart, Anthony A., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2003. |
| Description | vii, 63 leaves : illustrations (some color) ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Limb cartilage is derived from undifferentiated mesenchymal cells. Proteoglycans play an integral part in the extracellular matrix (ECM) and are important components of the developing limb. Versican is a chondroitin sulfate proteoglycan expressed by many developing tissues. Versican may be involved in limb chondrogenesis by interactions with cells and the ECM. Versican's role in cardiac development has been characterized in the heart defect (hdf) mouse. The hdf mouse contains an insertional mutation of the versican gene, interrupting expression of the mature proteoglycan. The hdf mouse serves as a viable model for versican's role in limb development. As the mutation is lethal in homozygotes by embryonic day 11 (before chondrogenic differentiation occurs) we developed a micromass tissue culture technique that prolonged forelimb viability from 10.5dpc embryos. Cartilage development in homozygous, heterozygous, and wild type embryos was compared to assess chondrogenic differences. Aggregation of the precartilage cells, expression of cartilage characteristic molecules, and versican presence were assayed using immunohistochemical and histochemical staining. Preliminary data indicated intense versican expression during aggregation. Statistical analysis of the wild type and heterozygous cultures appeared to support similar chondrogenic potential. Preliminary data indicated a lack of cartilage nodule formation in homozygous hdf embryos. Results of this study suggest that the mature versican proteoglycan is vital for aggregation during early stages of cartilage formation in embryonic limb development. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Anthony A. Capehart |
| Dissertation note | M.S. East Carolina University 2003 |
| Bibliography note | Includes bibliographical references (leaves 52-60). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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|---|---|---|---|---|
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| Electronic Resources | ✔ Available |