Comparative genomic analysis of the Morone saxatilis (striped bass) Hoxb4a gene : conservation and divergence of the Hoxb4 orthologs / by Robert M. Smith.
| Author/creator | Smith, Robert M. author. |
| Other author | Scemama, Jean-Luc, degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2003. |
| Description | 177 leaves : illustrations (some color) ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Identification, sequencing and analysis of several Hox genes from the striped bass (Morone saxatilis) has previously been completed as part of a comprehensive molecular analysis of the Morone Hox gene complex. Here, a single (lambda) EMBL-3 genomic clone 15.3kb in length from the striped bass was sequenced and characterized utilizing restriction mapping techniques, subcloning methods, automated DNA sequencing and DNA sequence analysis. DNA sequence comparisons of this generated sequence to other known Hox gene sequences revealed extensive DNA sequence similarity and distinctive amino acid and nucleotide signatures indicative of other Hoxb4 genes. Comparative analysis of the Morone non-protein coding DNA sequence to that of other vertebrate Hoxb4 gene regions revealed seven positionally conserved regions of high (50% to 97%) sequence similarity. These conserved regions of 100bp to 200bp in length were present in all six species used in the comparative analysis (including tetrapod mammals and other teleosts fishes) indicating that these regions are under selective pressure and therefore, are likely islands of potential regulatory regions/elements. Also identified were additional regions conserved among the teleost species only. These results demonstrate that, in general, the probable regulatory regions of the Hoxb4 gene orthologs have been well conserved in terms of many of the regulatory regions and the gene coding regions, in particular. These observations indicate that the identified regions, conserved across highly evolutionarily divergent species, most likely play critical roles in the regulation of the expression of the Hoxb4 genes of vertebrates. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Jean Luc Scemama |
| Dissertation note | M.S. East Carolina University 2003 |
| Bibliography note | Includes bibliographical references (leaves 98-105). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |