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003 OCoLC;

005 20250721152944.0;

008 040427s2003 xx a bm 000 0 eng d;

035 a| (Sirsi) o55029974;

035 a| (OCoLC)55029974;

049 a| EREE;

050 4 a| QP363.5b| .M368 2003;

100 1 a| McMurray, Roger J.,e| author.?| UNAUTHORIZED;

245 14 a| The effect of the selective estrogen receptor modulator LY117018 on peripheral nerve regeneration /c| by Roger J. McMurray.;

264 0 c| 2003.;

300 a| xv, 112 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2003;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Gerhard W. Kalmus;

500 a| Advisor: Alexander K. Murashov;

520 3 a| The aim of this study was to examine the effects of LY117018, a selective estrogen receptor modulator, on peripheral nerve regeneration, using a model of sciatic nerve crush injury in mice. Sciatic functional index, an index of functional recovery, was significantly higher in LY117018 treated mice throughout regeneration. Semi-thin sections revealed a significant increase in the total number of regenerating nerve fibers at day 7, and the mean axonal area of myelinated fibers at 7, 14, and 21 days after injury, in LY117018 treated mice. Immunohistochemistry revealed estrogen receptor-[alpha] and estrogen receptor-[beta] proteins localized in lumbar motor neurons. Quantitative real-time RT-PCR demonstrated an upregulation of estrogen receptors-[alpha] and -[beta] mRNA on the injured side of the lumbar spinal cord. Immunohistochemistry showed positive staining for estrogen receptor-[alpha] and -[beta], and extracellular signal-regulated kinase (ERK) in the axons of the sciatic nerve. Western blot confirmed the presence of these proteins in the nerve axons. Analysis of axonal transport through retrograde labeling of motor neurons showed that LY117018 potentiated axonal transport, while ICl 182,780 (inhibitor of estrogen receptors) and U0126 (inhibitor of ERK) blocked the action of LY117018, delineating estrogen receptors and ERK as its possible targets. These findings indicate that LY117018 markedly accelerated peripheral nerve regeneration and functional recovery, possibly via estrogen receptors and activation of the ERK signaling pathway.;

504 a| Includes bibliographical references (leaves 93-97).;

650 0 a| Nervous systemx| Regeneration.=| ^A94074;

650 0 a| Nerves, Peripheral.=| ^A5387;

650 0 a| Estrogenx| Receptors.=| ^A9694;

650 0 a| Mice as laboratory animals.=| ^A848;

650 2 a| Nerve Regeneration.0| (DNLM)D009416=| ^A920789;

650 2 a| Peripheral Nerves.0| (DNLM)D010525=| ^A948217;

650 2 a| Receptors, Estrogen.0| (DNLM)D011960=| ^A936323;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Estrogenx| Receptors.2| fast0| (OCoLC)fst00915646;

650 7 a| Mice as laboratory animals.2| fast0| (OCoLC)fst01019388;

650 7 a| Nerves, Peripheral.2| fast0| (OCoLC)fst01036068;

650 7 a| Nervous systemx| Regeneration.2| fast0| (OCoLC)fst01036139;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Kalmus, Gerhard W.,e| degree supervisor.?| UNAUTHORIZED;

700 1 a| Murashov, Alexander K.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12605;

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The effect of the selective estrogen receptor modulator LY117018 on peripheral nerve regeneration / by Roger J. McMurray.

Author/creator	McMurray, Roger J. author.
Other author	Kalmus, Gerhard W., degree supervisor.
Other author	Murashov, Alexander K., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2003.
Description	xv, 112 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Nervous system--Regeneration. Nerves, Peripheral. Estrogen--Receptors. Mice as laboratory animals. Nerve Regeneration. Peripheral Nerves. Receptors, Estrogen.

Summary	The aim of this study was to examine the effects of LY117018, a selective estrogen receptor modulator, on peripheral nerve regeneration, using a model of sciatic nerve crush injury in mice. Sciatic functional index, an index of functional recovery, was significantly higher in LY117018 treated mice throughout regeneration. Semi-thin sections revealed a significant increase in the total number of regenerating nerve fibers at day 7, and the mean axonal area of myelinated fibers at 7, 14, and 21 days after injury, in LY117018 treated mice. Immunohistochemistry revealed estrogen receptor-[alpha] and estrogen receptor-[beta] proteins localized in lumbar motor neurons. Quantitative real-time RT-PCR demonstrated an upregulation of estrogen receptors-[alpha] and -[beta] mRNA on the injured side of the lumbar spinal cord. Immunohistochemistry showed positive staining for estrogen receptor-[alpha] and -[beta], and extracellular signal-regulated kinase (ERK) in the axons of the sciatic nerve. Western blot confirmed the presence of these proteins in the nerve axons. Analysis of axonal transport through retrograde labeling of motor neurons showed that LY117018 potentiated axonal transport, while ICl 182,780 (inhibitor of estrogen receptors) and U0126 (inhibitor of ERK) blocked the action of LY117018, delineating estrogen receptors and ERK as its possible targets. These findings indicate that LY117018 markedly accelerated peripheral nerve regeneration and functional recovery, possibly via estrogen receptors and activation of the ERK signaling pathway.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Gerhard W. Kalmus
General note	Advisor: Alexander K. Murashov
Dissertation note	M.S. East Carolina University 2003
Bibliography note	Includes bibliographical references (leaves 93-97).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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