Characterization and expression of HOXB3a in Morone saxatilis / by Nykoll Lynn Williams.
| Author/creator | Williams, Nykoll Lynn author. |
| Other author | Scemama, Jean-Luc, degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 2001. |
| Description | x, 94 leaves : illustrations (some color) ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | The basic problem of developmental biology is to explain how a single cell can give rise to the many different cell types present in the adults. This process requires a complex developmental program in which specific genes are activated in a precise time sequence and in the correct location. Part of this process is directed by Hox genes, which are a subgroup of homeobox containing genes that are a developmentally regulated set of transcription factors present in a wide range of species that are responsible for regional specific tissue differentiation during development. Hox genes are highly related in sequence, but act very specifically in their roles in pattern formation and development. This study reports the characterization and expression of the Morone saxatilis HoxB3a gene. The complete genomic sequence, intron-exon junctions, and genomic comparisons are reported. HoxB3a is composed of two exons, separated by a single, large intron that follows the rules associated with splice sites. There is a region of homology between the Morone saxatilis, Fugu rubripes, Danio rerio, and Homo sapiens introns, but no known regulatory elements have been found in this region. The expression pattern of this gene is also analyzed throughout Morone saxatilis embryonic development using in situ hybridization. There are many similarities in the expression pattern of HoxB3a among diverse species. HoxB3a expression reported in teleosts, including striped bass, differs slightly from other organisms because the anterior limit of expression begins at the r4/r5 boundary and later moves anteriorly to the r3/r4 boundary. There is also a difference in the rhombomere with the most intense staining. |
| General note | Presented to the faculty of the Department of Biology. |
| General note | Advisor: Jean-Luc Scemama |
| Dissertation note | M.S. East Carolina University 2001 |
| Bibliography note | Includes bibliographical references (leaves 50-53). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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