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003 OCoLC;

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035 a| (Sirsi) o48651175;

035 a| (OCoLC)48651175;

049 a| EREE;

050 4 a| QH450b| .P37 2001;

100 1 a| Parker, Stephen Christopher James,e| author.?| UNAUTHORIZED;

245 10 a| Hox Gene clustering and regulatory networks /c| by Stephen Christopher James Parker.;

264 0 c| 2001.;

300 a| iv, 61 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2001;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Edmund J. Stellwag;

500 a| Advisor: Jean-Luc Scemama;

520 3 a| Hox genes have a very peculiar genomic architecture in that they are arranged in tightly linked clusters. Interestingly, this architecture has been maintained since the duplication of a single ancestral Hox cluster. Perhaps more intriguing is the colinear expression properties of Hox genes, wherein the anterior boundary and temporal sequence of expression of any particular gene is mirrored by its position within the cluster. A synthesis of comparative genomic and transgenic studies of Hox genes has led to the hypothesis that networks of cri-acting regulatory elements maintain the clustered architecture of Hox genes. There are two important corollaries to this hypothesis that will be addressed here. 1) Due to the critical nature of these regulatory elements one would expect them to be undergoing purifying selection in organisms with similar body plans. 2) The network of regulatory elements within each cluster should be extensive in order to account for the maintenance of the clustered architecture of Hox genes. In order to test these hypotheses I have developed a computational method to identify non-coding sequences under purifying selection. My results reveal multiple non-coding domains undergoing purifying selection within the Hoxba cluster of the teleost fish Morone saxatilis even in an area adjacent to a pseudogene. My results suggest that there is an extensive network of functional non-coding sequences throughout the Hox clusters, some which have been preferentially retained over coding sequences. I show that a previously identified non-coding sequence common to all vertebrate Hox clusters, herein referred to as the Gibraltar element (GBR), is under selection on clusters in which integrity is maintained but not when it is lost. I conclude that GBR may be required for Hox cluster stability and hypothesize that differential selective pressures applied to critical elements like the GBR may have been pivotal in the maintenance of clustered genetic networks in general and in the evolution of novel phenotypes.;

504 a| Includes bibliographical references (leaves 32-34).;

650 0 a| Genetic regulation.=| ^A6251;

650 0 a| Gene expression.=| ^A6250;

650 0 a| Gene mapping.=| ^A137648;

650 2 a| Gene Expression Regulation.0| (DNLM)D005786=| ^A920642;

650 2 a| Gene Expression.0| (DNLM)D015870=| ^A919546;

650 2 a| Chromosome Mapping.0| (DNLM)D002874=| ^A922405;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Gene expression.2| fast0| (OCoLC)fst00939613;

650 7 a| Gene mapping.2| fast0| (OCoLC)fst00939625;

650 7 a| Genetic regulation.2| fast0| (OCoLC)fst00940086;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Stellwag, Edmund J.q| (Edmund Johannes),d| 1951-e| degree supervisor.1| https://id.oclc.org/worldcat/entity/E39PCjxw8WYhVjjbHqyXbxhXMP=| ^A1031239;

700 1 a| Scemama, Jean-Luc,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12659;

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Hox Gene clustering and regulatory networks / by Stephen Christopher James Parker.

Author/creator	Parker, Stephen Christopher James author.
Other author	Stellwag, Edmund J. (Edmund Johannes), 1951- degree supervisor.
Other author	Scemama, Jean-Luc, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2001.
Description	iv, 61 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Genetic regulation. Gene expression. Gene mapping. Gene Expression Regulation. Gene Expression. Chromosome Mapping.

Summary	Hox genes have a very peculiar genomic architecture in that they are arranged in tightly linked clusters. Interestingly, this architecture has been maintained since the duplication of a single ancestral Hox cluster. Perhaps more intriguing is the colinear expression properties of Hox genes, wherein the anterior boundary and temporal sequence of expression of any particular gene is mirrored by its position within the cluster. A synthesis of comparative genomic and transgenic studies of Hox genes has led to the hypothesis that networks of cri-acting regulatory elements maintain the clustered architecture of Hox genes. There are two important corollaries to this hypothesis that will be addressed here. 1) Due to the critical nature of these regulatory elements one would expect them to be undergoing purifying selection in organisms with similar body plans. 2) The network of regulatory elements within each cluster should be extensive in order to account for the maintenance of the clustered architecture of Hox genes. In order to test these hypotheses I have developed a computational method to identify non-coding sequences under purifying selection. My results reveal multiple non-coding domains undergoing purifying selection within the Hoxba cluster of the teleost fish Morone saxatilis even in an area adjacent to a pseudogene. My results suggest that there is an extensive network of functional non-coding sequences throughout the Hox clusters, some which have been preferentially retained over coding sequences. I show that a previously identified non-coding sequence common to all vertebrate Hox clusters, herein referred to as the Gibraltar element (GBR), is under selection on clusters in which integrity is maintained but not when it is lost. I conclude that GBR may be required for Hox cluster stability and hypothesize that differential selective pressures applied to critical elements like the GBR may have been pivotal in the maintenance of clustered genetic networks in general and in the evolution of novel phenotypes.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Edmund J. Stellwag
General note	Advisor: Jean-Luc Scemama
Dissertation note	M.S. East Carolina University 2001
Bibliography note	Includes bibliographical references (leaves 32-34).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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