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035 a| (Sirsi) o46972899;

035 a| (OCoLC)46972899;

049 a| EREE;

050 4 a| QR414b| .L49 2000;

100 1 a| Lewis, Jennifer Aleisa,e| author.?| UNAUTHORIZED;

245 10 a| Persistent reovirus RNA, in the absence of detectable infectious reovirus, is associated with the progression of biliary atresia in neonatal mice /c| by Jennifer Aleisa Lewis.;

264 0 c| 2000.;

300 a| 78 leaves :b| illustrations (some color) ;c| 29 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2000;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Stephanie Oberhaus;

520 3 a| Extrahepatic biliary atresia (EHBA) is a serious disease causing blockage of bile ducts, liver damage, and eventually death in young children. The cause of EHBA is unknown and the only two available treatments are costly and not highly effective. Reovirus serotype 3 infection of neonatal mice has been shown to produce symptoms like those of human EHBA, and is a well-characterized system for studying viral pathogenesis, identifying it as a potentially useful animal system in which to study this disease. Reovirus has been shown to induce apoptosis in mouse tissues, suggesting it as a mechanism for tissue destruction and the production of disease. We have previously shown that, following peroral inoculation of with reovirus T3A, mice with biliary atresia have significant tissue damage and apoptosis localized to portal triad regions of the liver after peak viral titer has been reached, and often when infectious virus can no longer be detected by immunofluorescence or in situ hybridization. Using reverse transcriptase-polymerase chain reaction (RT-PCR), others have detected reovirus in the livers of mice 50 days post-infection, long after infectious virus is no longer detectable. In order to determine whether or not reovirus RNA persisted in the livers of mice with biliary atresia, in the absence of detectable infectious virus, a nested RT-PCR was used for detecting reovirus LI RNA. Reovirus LI RNA was found in the absence of detectable infectious virus, when liver tissue damage and apoptosis associated with biliary atresia were at their peak. These data suggest that persistent reovirus RNA is associated with the progression of this disease in mice.;

504 a| Includes bibliographical references (leaves 73-78).;

650 0 a| Reoviruses.=| ^A28348;

650 0 a| RNA viruses.=| ^A3756;

650 0 a| Biliary atresia.=| ^A470161;

650 0 a| Mice as laboratory animals.=| ^A848;

650 2 a| Reoviridae.0| (DNLM)D012087=| ^A924407;

650 2 a| RNA Viruses.0| (DNLM)D012328=| ^A948527;

650 2 a| Biliary Atresia.0| (DNLM)D001656=| ^A1480217;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Biliary atresia.2| fast0| (OCoLC)fst00831712;

650 7 a| Reoviruses.2| fast0| (OCoLC)fst01094755;

650 7 a| RNA viruses.2| fast0| (OCoLC)fst01086258;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Oberhaus, Stephanie M.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12567;

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994 a| C0b| ERE;

596 a| 1 4;

998 a| 801831;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 1341180d| 5/29/2001l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 5/21/2001x| ETDz| JSPECCOLLo| .STAFF. 0;

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Persistent reovirus RNA, in the absence of detectable infectious reovirus, is associated with the progression of biliary atresia in neonatal mice / by Jennifer Aleisa Lewis.

Author/creator	Lewis, Jennifer Aleisa author.
Other author	Oberhaus, Stephanie M., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2000.
Description	78 leaves : illustrations (some color) ; 29 cm
Supplemental Content	Access via ScholarShip
Subjects	Reoviruses. RNA viruses. Biliary atresia. Mice as laboratory animals. Reoviridae. RNA Viruses. Biliary Atresia.

Summary	Extrahepatic biliary atresia (EHBA) is a serious disease causing blockage of bile ducts, liver damage, and eventually death in young children. The cause of EHBA is unknown and the only two available treatments are costly and not highly effective. Reovirus serotype 3 infection of neonatal mice has been shown to produce symptoms like those of human EHBA, and is a well-characterized system for studying viral pathogenesis, identifying it as a potentially useful animal system in which to study this disease. Reovirus has been shown to induce apoptosis in mouse tissues, suggesting it as a mechanism for tissue destruction and the production of disease. We have previously shown that, following peroral inoculation of with reovirus T3A, mice with biliary atresia have significant tissue damage and apoptosis localized to portal triad regions of the liver after peak viral titer has been reached, and often when infectious virus can no longer be detected by immunofluorescence or in situ hybridization. Using reverse transcriptase-polymerase chain reaction (RT-PCR), others have detected reovirus in the livers of mice 50 days post-infection, long after infectious virus is no longer detectable. In order to determine whether or not reovirus RNA persisted in the livers of mice with biliary atresia, in the absence of detectable infectious virus, a nested RT-PCR was used for detecting reovirus LI RNA. Reovirus LI RNA was found in the absence of detectable infectious virus, when liver tissue damage and apoptosis associated with biliary atresia were at their peak. These data suggest that persistent reovirus RNA is associated with the progression of this disease in mice.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Stephanie Oberhaus
Dissertation note	M.S. East Carolina University 2000
Bibliography note	Includes bibliographical references (leaves 73-78).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Persistent reovirus RNA, in the absence of detectable infectious reovirus, is associated with the progression of biliary atresia in neonatal mice / by Jennifer Aleisa Lewis.

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