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LEADER 03461ctm a2200541 i 4500

001 ocm46961575;

003 OCoLC;

005 20260722082103.0;

008 010517s2000 xx a bm 000 0 eng d;

035 a| (Sirsi) o46961575;

035 a| (OCoLC)46961575;

049 a| EREE;

050 4 a| QH442.2b| .S65 2000;

100 1 a| Smith, April W.,e| author.1| https://id.oclc.org/worldcat/entity/E39PCjxykHjqFBPTdVgVjHj8cq=| ^A476305;

245 10 a| Cloning of the 28S ribosomal DNA gene in the gray tree frog, hyla chrysoscelis /c| by April W. Smith.;

264 0 c| 2000.;

300 a| 33 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 2000;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: John E. Wiley;

520 3 a| The 18S and 28S ribosomal genes are usually found in the same chromosome location within a species. However, rDNA genes have been shown to be polymorphic for chromosome location in a number of species, including Hyla chrysoscelis. The mechanism by which these genes 'jump' from one chromosome to another is unknown. Sequencing the ribosomal gene array is the first step towards understanding the nature of this polymorphism. A 3.7 Kb PCR product was generated, cloned and sequenced. BLAST analysis revealed that the product had extensive homology to the 28S rDNA gene of many species, with the greatest homology to Xenopus laevis. Fluorescent in situ hybridization using the cloned PCR product as a probe and pXlr11, a recombinant plasmid derived from Xenopus laevis, as the control probe showed hybridization signal on the same chromosomes and in the same loci with both probes. Approximately 1200 bases have been sequenced; the first 179 bases of the 5' end and 1033 bases near the 3' terminus of the gene.;

504 a| Includes bibliographical references (leaves 31-33).;

650 0 a| Cloning.=| ^A8574;

650 0 a| DNAx| Analysis.=| ^A13543;

650 0 a| Hylidae.=| ^A358053;

650 2 a| Cloning, Organism.0| (DNLM)D019976=| ^A932125;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Cloning.2| fast0| (OCoLC)fst00864579;

650 7 a| DNAx| Analysis.2| fast0| (OCoLC)fst00886556;

650 7 a| Hylidae.2| fast0| (OCoLC)fst00965432;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Wiley, John E.,e| degree supervisor.1| https://id.oclc.org/worldcat/entity/E39PCjrFB7HWC7yyxf7B7FvQC3=| ^A788626;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12760;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 801602;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 1340900d| 5/29/2001l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 5/17/2001x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 801602-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 5/28/2026x| ETDz| JERESOURCE;

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Cloning of the 28S ribosomal DNA gene in the gray tree frog, hyla chrysoscelis / by April W. Smith.

Author/creator	Smith, April W. author.
Other author	Wiley, John E., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	2000.
Description	33 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Cloning. DNA--Analysis. Hylidae. Cloning, Organism.

Summary	The 18S and 28S ribosomal genes are usually found in the same chromosome location within a species. However, rDNA genes have been shown to be polymorphic for chromosome location in a number of species, including Hyla chrysoscelis. The mechanism by which these genes 'jump' from one chromosome to another is unknown. Sequencing the ribosomal gene array is the first step towards understanding the nature of this polymorphism. A 3.7 Kb PCR product was generated, cloned and sequenced. BLAST analysis revealed that the product had extensive homology to the 28S rDNA gene of many species, with the greatest homology to Xenopus laevis. Fluorescent in situ hybridization using the cloned PCR product as a probe and pXlr11, a recombinant plasmid derived from Xenopus laevis, as the control probe showed hybridization signal on the same chromosomes and in the same loci with both probes. Approximately 1200 bases have been sequenced; the first 179 bases of the 5' end and 1033 bases near the 3' terminus of the gene.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: John E. Wiley
Dissertation note	M.S. East Carolina University 2000
Bibliography note	Includes bibliographical references (leaves 31-33).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Cloning of the 28S ribosomal DNA gene in the gray tree frog, hyla chrysoscelis / by April W. Smith.

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