Cloning of the 18s rDNA gene, an internal transcribed spacer, and the 5' region of the 28s rDNA gene of the gray treefrog, Hyla chrysoscelis / by Gary Owens, Jr.
| Author/creator | Owens, Gary author. |
| Other author | Wiley, John E., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1999. |
| Description | iv, 58 leaves : illustrations (some color) ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | The class one transcription unit contains the rDNA genes responsible for the synthesis of ribosomes. The unit is composed of a tandem repeat of the following: an external transcribed spacer, the 18s rDNA gene, an internal transcribed spacer (ITS), the 5.8S rDNA gene, another ITS, the 28S rDNA gene, and a non-transcribed intergenic region. The location of rDNA genes on chromosomes in most species is identical within that species, usually occurring on the same chromosome or chromosomes. This statement has been found to be untrue in Hyla chrysocelis, the Gray Treefrog. The rDNA genes in populations of Hyla chrysocelis are polymorphic for chromosomal location and are found on several different chromosomes. The occasions leading to these polymorphisms have yet to be determined. Possible explanations for polymorphisms include translocations, inversions, mobile genetic elements, or a combination of these. The first step in discovering how these genes become polymorphic is the isolation of the genes. Primers for the Polymerase Chain Reaction were created using data from multiple sequence alignments of conserved rDNA gene sequences and the Genetics Computer Group (GCG) Prime package. The 18S rDNA gene and a region including the end of the 18S rDNA gene, an internal transcribed spacer, and a portion of the 5' end of the 28S rDNA gene were amplified using PCR. These PCR products were inserted into the pCRII-TOPO plasmid, and cloned. The clones obtained were confirmed by fluorescent in-situ hybridization (FISH), and sequenced using an Applied Biosystems Automated DNA Sequencer. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: John E. Wiley |
| Dissertation note | M.S. East Carolina University 1999 |
| Bibliography note | Includes bibliographical references (leaves 55-58). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |