Methods for imaging cell membranes / Dylan Owen, Luca Panconi, Daniel Nieves, Maria Makarova.
| Author/creator | Owen, Dylan M. |
| Other author | Panconi, Luca |
| Other author | Nieves, Daniel |
| Other author | Makarova, Maria, Dr. |
| Format | Electronic |
| Edition | First edition. |
| Publication Info | Boca Raton, FL : CRC Press/Taylor & Francis Group, 2024. |
| Description | 125 pages color illustrations 25 cm |
| Supplemental Content | Full text available from eBooks on EBSCOhost |
| Subjects |
| Physical medium | illustrations |
| Physical medium | color |
| Contents | General introduction to imaging the plasma membrane -- How to map membrane proteins at the single-molecule level -- Measuring diffusion of membrane biomolecules using fluorescence microscopy -- Detecting membrane protein dimers and oligomers by FRET -- Imaging membrane biophysical properties -- Conclusions and future perspectives. |
| Abstract | "This book will serve as an introduction to microscopy and biomedical imaging methods, focussed on the study of the distributions and dynamics of molecules on the cell surface. It will provide readers with an in-depth understanding of how modern microscopy methodology can be used to understand the organisation of cell membrane systems and how experiments can be designed around these methodologies. There are numerous methods employed to understand cell membrane organisation, but foremost among them are microscopy methods which can map the distributions of molecules on the cell surface and even map the biophysical properties of membranes themselves. Fluorescence microscopy has been especially widely used due to its specificity and relatively non-invasive nature, allowing live-cell imaging. However, the recent advance of super-resolution fluorescence microscopy has broken the previous resolution limit for this type of microscopy has been an important advancement in the field. Atomic force microscopy and electron microscopy have also been deployed to learn about membrane organisation and properties. Each chapter in this volume will be themed around measuring a particular property of cell membranes. In each case the authors examine the range of methodology applicable to the task, comparing the advantages and disadvantages of each one, and will also provide an overview of important discoveries which have been made using the methodology being discussed"-- Provided by publisher. |
| Bibliography note | Includes bibliographical references and index. |
| Access restriction | Available only to authorized users. |
| Technical details | Mode of access: World Wide Web |
| Genre/form | Electronic books. |
| LCCN | 2023030749 |
| ISBN | 9781032207902 (hardback) |
| ISBN | 9781032226941 (paperback) |
| ISBN | (ebook) |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Electronic Resources | Access Content Online | ✔ Available |