Immunohistochemistry : a technical guide to current practices / edited by Trung Nguyen.

Other author Nguyen, Trung, 1976- editor.
Format Electronic
PublicationCambridge, United Kingdom ; New York, NY : Cambridge University Press, 2022.
Copyright Date©2022
Description1 online resource (x, 272 pages) : color illustrations
Supplemental ContentDirect link to eBook
Subjects

Uniform titleImmunohistochemistry (Nguyen)
Contents Immunohistochemistry fundamentals / Enisa Hasic -- Immunohistochemistry quality assurance and quality control / Bronwyn Christiansen & Hazel Chambers -- Automation and robotics with the Leica Bond III / Anthony F. Henwood -- Automation and robotics with the Roche ventana benchmark ULTRA -- Automation and robotics with the Agilent dako omnis / Andrew Dinan -- Immunohistochemistry for research applications / Trung Nguyen -- Troubleshooting immunohistochemistry / David Gan -- Current status of immunohistochemistry / Trung Nguyen -- Immunohistochemistry for future applications / Candice J Clarke.
Abstract "This book directs the reader to areas within IHC that are important to know with ideas that are proven to work in real world settings. Special emphasis is made to lean and efficient principles. Both the theoretical and the practical are covered, written by authors who are current practising medical scientists in diagnostic histology laboratories. These contributors have made all the mistakes, worked at the coalface of IHC and have successfully troubleshooted their way out of many predicaments over the years. They are experts in this field of medical science and are willing to share their collective experiences with the readership. Throughout the book, common themes emerge. Validation is the method of selecting suitable antibody clones, and the foundation to successful IHC is adherence to the principles of pre-analytic, analytic and post-analytic standardisation. A major emphasis is placed on why IHC assays fail. Protocols can be quite technical or become unnecessarily complicated, and there are numerous traps to avoid for the inexperienced investigator. In gaining a deeper understanding of the technology, it is hoped that the laboratory worker can consistently achieve a clean, crisp and optimised IHC stain"-- Provided by publisher.
Bibliography noteIncludes bibliographical references and index.
Source of descriptionDescription based on online resource; title from digital title page (viewed on June 24, 2022).
Issued in other formPrint version: Immunohistochemistry (Nguyen) Immunohistochemistry Cambridge, United Kingdom ; New York, NY : Cambridge University Press, 2022 9781009107723
LCCN 2021047524
ISBN9781009106924 electronic book
ISBN1009106929 electronic book
ISBNpaperback

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Electronic Resources Access Content Online ✔ Available