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035 a| (Sirsi) o1523697749;

035 a| (OCoLC)1523697749;

049 a| EREE;

090 a| RC280.C6;

100 1 a| Ochoa, Carlos A,e| author.?| UNAUTHORIZED;

245 10 a| Investigating the role of pH-sensing G protein-coupled receptors GPR4 and GPR132 in colorectal cancer :b| multi-statistical, survival, and structural analysis approach /c| by Carlos A Ochoa.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2025.;

300 a| 1 online resource (52 pages) :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| application/pdfc| 1.19 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2025.;

500 a| Presented to the Faculty of the Department of Computer Science.;

500 a| Advisor: Qin Ding;

500 a| Title from PDF t.p. (viewed June 10, 2026).;

520 3 a| Colorectal adenocarcinoma (COAD) is one of the leading causes of cancer-related mortality worldwide. Understanding the molecular mechanisms that contribute to cancer progression is a critical step for identifying therapeutic targets to combat COAD. GPR4 and GPR132, which are pH sensing G protein-coupled receptors (GPCRs), have recently emerged as a point of interest and linked to tumor progression, tumor microenvironment, and molecular signaling pathways. However, despite this, the roles these GPCRs play are still not fully understood. This study investigates the clinical and structural relevance of GPR4 and GPR132 in COAD through gene expression, survival, and structural analyses. Gene expression and clinical data were collected from The Cancer Genome Atlas (TCGA) and analyzed using various statistical and survival methods. Statistical tests and survival models revealed an increase in GPR4 expression and that higher stages were significantly associated with worsening patient survival outcomes, which suggests GPR4 to be a potential biomarker and therapeutic target. In contrast GPR132 showed a limited amount of clinical significance and was hindered by a lack of comprehensive clinical data. Additionally, AlphaFold and APBS were used to model wildtype and mutation GPR4 structures and electrostatic potential (ESP) maps across different pH levels. While electrostatic differences were inconclusive and need further in-depth investigation, structural comparisons discovered notable spatial changes between the transmembrane domain that contains position 115 and two other transmembrane domains. Overall, this study highlights prognostic potential in COAD and provides preliminary insights into how mutations may influence its structure and function.;

504 a| Includes bibliographical references.;

650 0 a| Colon (Anatomy)x| Cancer.=| ^A230293;

650 0 a| G proteinsx| Receptors.=| ^A807660;

650 0 a| Bioinformatics.=| ^A493304;

653 a| GPCR's;

653 a| Computer Science;

700 1 a| Ding, Qin,e| degree supervisor.=| ^A1464811;

710 2 a| East Carolina University.b| Department of Computer Science.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/14099;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6584088;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6584088-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/16/2025x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6584088-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/16/2025x| ETDz| HERESOURCE;

Investigating the role of pH-sensing G protein-coupled receptors GPR4 and GPR132 in colorectal cancer : multi-statistical, survival, and structural analysis approach / by Carlos A Ochoa.

Author/creator	Ochoa, Carlos A author.
Other author	Ding, Qin, degree supervisor.
Other author	East Carolina University. Department of Computer Science.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2025.
Description	1 online resource (52 pages) : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	Colon (Anatomy)--Cancer. G proteins--Receptors. Bioinformatics.

Summary	Colorectal adenocarcinoma (COAD) is one of the leading causes of cancer-related mortality worldwide. Understanding the molecular mechanisms that contribute to cancer progression is a critical step for identifying therapeutic targets to combat COAD. GPR4 and GPR132, which are pH sensing G protein-coupled receptors (GPCRs), have recently emerged as a point of interest and linked to tumor progression, tumor microenvironment, and molecular signaling pathways. However, despite this, the roles these GPCRs play are still not fully understood. This study investigates the clinical and structural relevance of GPR4 and GPR132 in COAD through gene expression, survival, and structural analyses. Gene expression and clinical data were collected from The Cancer Genome Atlas (TCGA) and analyzed using various statistical and survival methods. Statistical tests and survival models revealed an increase in GPR4 expression and that higher stages were significantly associated with worsening patient survival outcomes, which suggests GPR4 to be a potential biomarker and therapeutic target. In contrast GPR132 showed a limited amount of clinical significance and was hindered by a lack of comprehensive clinical data. Additionally, AlphaFold and APBS were used to model wildtype and mutation GPR4 structures and electrostatic potential (ESP) maps across different pH levels. While electrostatic differences were inconclusive and need further in-depth investigation, structural comparisons discovered notable spatial changes between the transmembrane domain that contains position 115 and two other transmembrane domains. Overall, this study highlights prognostic potential in COAD and provides preliminary insights into how mutations may influence its structure and function.
General note	Presented to the Faculty of the Department of Computer Science.
General note	Advisor: Qin Ding
General note	Title from PDF t.p. (viewed June 10, 2026).
Dissertation note	M.S. East Carolina University 2025.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Investigating the role of pH-sensing G protein-coupled receptors GPR4 and GPR132 in colorectal cancer : multi-statistical, survival, and structural analysis approach / by Carlos A Ochoa.

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