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035 a| (OCoLC)1456021965;

090 a| TD745;

049 a| EREE;

100 1 a| Cobb, Patrick "Davis",e| author.?| UNAUTHORIZED;

245 10 a| Electrochemical detection of polyphosphate accumulation by phosphate accumulating organisms for wastewater treatment /c| by Patrick "Davis" Cobb.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2024.;

300 a| 1 online resource (51 pages) :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| application/pdfc| 3.61 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2024.;

500 a| Presented to the Faculty of the Department of Chemistry;

500 a| Advisor: Eli Hvastkovs;

500 a| Title from PDF t.p. (viewed February 13, 2026).;

520 3 a| Inorganic phosphates are a very common additive to fertilizers used in industrial agriculture. While these additions result in larger healthier crop yields the runoff from these macronutrients ends up within local water ecosystems. The discharge of phosphate to water systems can lead to eutrophication and long-term damage to the local environment. To treat phosphate, local wastewater treatment systems (WWTP) often use bacteria to remove phosphate from the water. These phosphate treatment organisms are termed phosphate accumulating organisms (PAO) and sequester phosphate by converting it into long chains of polyphosphate. The efficiency of this process can vary due to myriad factors; therefore, the ability to monitor if the biological processes are occurring normally is a significant WWTP need. To this end, an electrochemical biosensor was developed using Layer-by-Layer (LbL) methodology to immobilize films containing Pseudomonas putida, a model PAO, to detect polyphosphate uptake following soluble phosphate exposure. The bacterial films were exposed to electroactive methylene blue and phosphate with electrochemical monitoring using square wave voltammetry to determine methylene blue reduction currents. Currents at several potentials (vs. SCE) increase as a function of phosphate exposure, which were significantly elevated in the presence of P. putida vs. E. coli films. ICP-MS was used to show that P. putida sequesters phosphorus in significantly higher amounts than E. coli, which shows that the electrochemical output is due to poly-P accumulation on the electrode surface. The sensor was challenged with interfering ion solutions and at varying temperatures to model conditions that might be encountered at the WWTP. Overall, this study lays the groundwork for the eventual biosensor use to measure PAO health and function at WWTP.;

504 a| Includes bibliographical references.;

650 0 a| Sewagex| Purificationx| Biological treatment.=| ^A4110;

650 0 a| Phosphatic fertilizersx| Control.=| ^A1012164;

650 0 a| Sewage disposal plants.=| ^A14089;

650 0 a| Biosensors.=| ^A149319;

650 7 a| sewage treatment plants.2| aat0| (CStmoGRI)aat300006218;

653 a| Wastewater;

700 1 a| Hvastkovs, Eli G.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Chemistry.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/13719;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6477474;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6477474-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/16/2024x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6477474-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/16/2024x| ETDz| HERESOURCE;

Electrochemical detection of polyphosphate accumulation by phosphate accumulating organisms for wastewater treatment / by Patrick "Davis" Cobb.

Author/creator	Cobb, Patrick "Davis" author.
Other author	Hvastkovs, Eli G., degree supervisor.
Other author	East Carolina University. Department of Chemistry.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2024.
Description	1 online resource (51 pages) : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Sewage--Purification. --Biological treatment. Phosphatic fertilizers--Control. Sewage disposal plants. Biosensors.

Summary	Inorganic phosphates are a very common additive to fertilizers used in industrial agriculture. While these additions result in larger healthier crop yields the runoff from these macronutrients ends up within local water ecosystems. The discharge of phosphate to water systems can lead to eutrophication and long-term damage to the local environment. To treat phosphate, local wastewater treatment systems (WWTP) often use bacteria to remove phosphate from the water. These phosphate treatment organisms are termed phosphate accumulating organisms (PAO) and sequester phosphate by converting it into long chains of polyphosphate. The efficiency of this process can vary due to myriad factors; therefore, the ability to monitor if the biological processes are occurring normally is a significant WWTP need. To this end, an electrochemical biosensor was developed using Layer-by-Layer (LbL) methodology to immobilize films containing Pseudomonas putida, a model PAO, to detect polyphosphate uptake following soluble phosphate exposure. The bacterial films were exposed to electroactive methylene blue and phosphate with electrochemical monitoring using square wave voltammetry to determine methylene blue reduction currents. Currents at several potentials (vs. SCE) increase as a function of phosphate exposure, which were significantly elevated in the presence of P. putida vs. E. coli films. ICP-MS was used to show that P. putida sequesters phosphorus in significantly higher amounts than E. coli, which shows that the electrochemical output is due to poly-P accumulation on the electrode surface. The sensor was challenged with interfering ion solutions and at varying temperatures to model conditions that might be encountered at the WWTP. Overall, this study lays the groundwork for the eventual biosensor use to measure PAO health and function at WWTP.
General note	Presented to the Faculty of the Department of Chemistry
General note	Advisor: Eli Hvastkovs
General note	Title from PDF t.p. (viewed February 13, 2026).
Dissertation note	M.S. East Carolina University 2024.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Electrochemical detection of polyphosphate accumulation by phosphate accumulating organisms for wastewater treatment / by Patrick "Davis" Cobb.

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