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035 a| (OCoLC)1456021953;

090 a| QP801.C27;

049 a| EREE;

100 1 a| Tunnell, Heather,e| author.?| UNAUTHORIZED;

245 10 a| Identification and quantitation of cannabinoid solute-column interactions using the hydrophobic-subtraction model to predict separation in high-performance liquid chromatography /c| by Heather Tunnell.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2024.;

300 a| 1 online resources (74 pages) :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| application/pdfc| 1.26 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2024.;

500 a| Presented to the Faculty of the Department of Chemistry;

500 a| Advisor: Jack Pender;

500 a| Title from PDF t.p. (viewed January 16, 2026).;

520 3 a| The Hydrophobic-Subtraction Model (HSM) has been widely used for identification of similar and orthogonal chromatography columns since its inception in 2002 but only had limited uses as a predictive tool. Recent refinement of the model has improved the prediction accuracy to a level that offers promise for use in high-performance liquid chromatography (HPLC) separation development. The HSM quantitatively describes the parameters affecting solute retention on HPLC columns including hydrophobic, steric, hydrogen-bond, cation exchange, and dipole interactions. To demonstrate the process, 16 cannabinoid compounds were chosen as the test solutes due to increasing interest and applications. The HSM was used to identify and quantify the various cannabinoid solute retention parameters based on actual retention of each solute on a set of calibration columns with previously quantified column retention parameters. The resulting solute parameters were used to predict relative retention of each cannabinoid on over 550 columns in the HSM column database. A sorting system was then developed to rank the columns. Overall, this Thesis demonstrates the application of the HSM to cannabinoid compounds starting with identification of the best columns for the separation of these solutes.;

504 a| Includes bibliographical references.;

650 0 a| Cannabinoids.=| ^A828277;

650 0 a| Liquid chromatography.=| ^A6409;

650 0 a| Solution (Chemistry)=| ^A6084;

650 7 a| liquid chromatography.2| aat0| (CStmoGRI)aat300214724;

650 7 a| solution.2| aat0| (CStmoGRI)aat300210311;

653 a| Hydrophobic-Subtraction Model;

653 a| HPLC;

653 a| Columns;

653 a| Selectivity;

700 1 a| Pender, Jack,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Chemistry.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/13724;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6477470;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6477470-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/16/2024x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6477470-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/16/2024x| ETDz| HERESOURCE;

Identification and quantitation of cannabinoid solute-column interactions using the hydrophobic-subtraction model to predict separation in high-performance liquid chromatography / by Heather Tunnell.

Author/creator	Tunnell, Heather author.
Other author	Pender, Jack, degree supervisor.
Other author	East Carolina University. Department of Chemistry.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2024.
Description	1 online resources (74 pages) : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Cannabinoids. Liquid chromatography. Solution (Chemistry)

Summary	The Hydrophobic-Subtraction Model (HSM) has been widely used for identification of similar and orthogonal chromatography columns since its inception in 2002 but only had limited uses as a predictive tool. Recent refinement of the model has improved the prediction accuracy to a level that offers promise for use in high-performance liquid chromatography (HPLC) separation development. The HSM quantitatively describes the parameters affecting solute retention on HPLC columns including hydrophobic, steric, hydrogen-bond, cation exchange, and dipole interactions. To demonstrate the process, 16 cannabinoid compounds were chosen as the test solutes due to increasing interest and applications. The HSM was used to identify and quantify the various cannabinoid solute retention parameters based on actual retention of each solute on a set of calibration columns with previously quantified column retention parameters. The resulting solute parameters were used to predict relative retention of each cannabinoid on over 550 columns in the HSM column database. A sorting system was then developed to rank the columns. Overall, this Thesis demonstrates the application of the HSM to cannabinoid compounds starting with identification of the best columns for the separation of these solutes.
General note	Presented to the Faculty of the Department of Chemistry
General note	Advisor: Jack Pender
General note	Title from PDF t.p. (viewed January 16, 2026).
Dissertation note	M.S. East Carolina University 2024.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Identification and quantitation of cannabinoid solute-column interactions using the hydrophobic-subtraction model to predict separation in high-performance liquid chromatography / by Heather Tunnell.

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