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LEADER 03241ctm a2200481 i 4500

001 ocm38987384;

003 OCoLC;

005 20240729154354.0;

008 980421s1996 xx a bm 000 0 eng d;

035 a| (Sirsi) o38987384;

035 a| (OCoLC)38987384;

049 a| EREE;

050 4 a| QL638.13b| .F56 1996;

100 1 a| Flores, Michael,e| author.=| ^A1225271;

245 10 a| Characterization of the MHC class II A genes of the channel catfish, ictalurus punctatus /c| by Michael Flores.;

264 0 c| 1996.;

300 a| 88 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1996;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Thomas J. McConnell;

520 3 a| The Major Histocompatihility Complex (Mhc) class II A genes in the channel catfish (Ictalurus punctatus) were isolated utilizing cDNA libraries and polymerase chain reaction techniques. Two unique clones were isolated from a cDNA library using striped bass and channel catfish Mhc class II A probes. Upon sequence analysis, the two clones were found to have a high degree of homology with the class II A genes from zebrafish and several other teleosts. Primers were designed and two additional clones were isolated by PCR+1. Comparison with other Mhc encoding genes revealed that the products contained the entire coding region. The identification of four different class II A gene sequences in a diploid organism suggests the presence of at least two loci, results that are supported by Southern blot analysis. Southern blot analysis also confirmed a greater degree of polymorphism in the B genes as opposed to the A genes. When the four gene sequences were translated, each contained several structural motifs that would indicate function, including the conserved cysteines within the a1 and a2 domains.;

504 a| Includes bibliographical references (leaves 80-88).;

650 0 a| Channel catfishx| Genetics.=| ^A112918;

650 7 a| Channel catfishx| Genetics.2| fast0| (OCoLC)fst00852109;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| McConnell, Thomas J.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/11983;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 629348;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 1124494d| 12/3/2001l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 12/3/2001x| ETDz| JSPECCOLLo| .STAFF. 0;

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Characterization of the MHC class II A genes of the channel catfish, ictalurus punctatus / by Michael Flores.

Author/creator	Flores, Michael author.
Other author	McConnell, Thomas J., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1996.
Description	88 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Channel catfish--Genetics.

Summary	The Major Histocompatihility Complex (Mhc) class II A genes in the channel catfish (Ictalurus punctatus) were isolated utilizing cDNA libraries and polymerase chain reaction techniques. Two unique clones were isolated from a cDNA library using striped bass and channel catfish Mhc class II A probes. Upon sequence analysis, the two clones were found to have a high degree of homology with the class II A genes from zebrafish and several other teleosts. Primers were designed and two additional clones were isolated by PCR+1. Comparison with other Mhc encoding genes revealed that the products contained the entire coding region. The identification of four different class II A gene sequences in a diploid organism suggests the presence of at least two loci, results that are supported by Southern blot analysis. Southern blot analysis also confirmed a greater degree of polymorphism in the B genes as opposed to the A genes. When the four gene sequences were translated, each contained several structural motifs that would indicate function, including the conserved cysteines within the a1 and a2 domains.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Thomas J. McConnell
Dissertation note	M.S. East Carolina University 1996
Bibliography note	Includes bibliographical references (leaves 80-88).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Characterization of the MHC class II A genes of the channel catfish, ictalurus punctatus / by Michael Flores.

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