The effects of two bouts of eccentric exercise on insulin sensitivity, glycogen repletion, and GLUT4 proteins / by Natalie E. Lew.
| Author/creator | Lew, Natalie E. author. |
| Other author | Hickey, Matthew S., degree supervisor. |
| Other author | East Carolina University. Department of Kinesiology. |
| Format | Theses and dissertations |
| Production | 1996. |
| Description | vii, 98 leaves : illustrations, forms ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | The purpose of this study was to determine insulin sensitivity and GLUT4 protein concentrations 48 hours following an initial bout of damage-inducing single leg isokinetic eccentric exercise, and 48 hours after a repeated bout of single leg isokinetic eccentric exercise performed 14 days after the initial bout. Thirteen active but untrained Caucasian males volunteered for this study. No subject had performed any weight training during the previous six months. Following screening and completion of the informed consent, each subject was randomly assigned to exercise either the right or left leg. Subjects performed two exercise bouts spaced 14 days apart. The exercise session consisted of 12 sets of 10 eccentric knee flexions (quadriceps) and 10 sets of 10 eccentric knee extensions (hamstrings). Approximately 5 days prior to the initial exercise bout and approximately 48 hours after each exercise bout, the subjects completed a 3 hour Intravenous Glucose Tolerance Test (IVGTT). Subjects consumed a controlled diet during the 48 hours prior to each 3 hour IVGTT and fasted for twelve hours prior to each IVGTT. Blood samples were analyzed for insulin and glucose and used to determine insulin sensitivity using the minimal model method. In addition, the serum baseline blood sample from each IVGTT was analyzed for creatine kinase (CK). Within the last 30 minutes of each 3 hour IVGTT, a muscle biopsy was taken from the vastus lateralis of the exercise and control legs. The muscle samples were analyzed for GLUT4 proteins. The initial intention was to analyze tissue samples for glycogen; however, due to methodological problems sufficient tissue was not available. For both exercise bouts, Delayed Onset Muscle Soreness (DOMS) was assessed pre-exercise and at 24, 48, 72, 96, and 120 hours post-exercise. Data were analyzed using a repeated measures ANOVA. DOMS, maximum torque, and creatine kinase responses suggested that the eccentric exercise routine elicited changes consistent with a single and repeated bout of eccentric exercise. However, there was no significant difference in ISI between control, bout 1, and bout 2 (p > 0.05). GLUT-4 was only measured on four subjects. No significant effects (p > 0.05) were found for bout (1 versus 2), treatment (control versus exercise leg), or bout by treatment. Thus, this study demonstrated no significant increases or decreases in insulin sensitivity index, suggesting that single leg isokinetic eccentric exercise does not alter ISI when assessed using an IVGTT. In addition, this study demonstrated no significant change in GLUT4 protein concentration measured on four subjects, suggesting that single leg isokinetic eccentric exercise does not alter GLUT4 protein concentrations when compared to baseline concentrations of either the exercised or non-exercised leg. |
| General note | Submitted to the faculty of the Department of Exercise and Sport Science. |
| General note | Advisor: Matthew S. Hickey |
| Dissertation note | M.A. East Carolina University 1996 |
| Bibliography note | Includes bibliographical references (leaves 62-71). |
| Genre/form | masters theses. |
| Genre/form | theses. |
| Genre/form | dissertations. |
| Genre/form | doctoral dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
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|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |