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003 OCoLC;

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035 a| (OCoLC)1397376849;

090 a| RC280.B8;

049 a| EREE;

100 1 a| Libby, Nichole,e| author.?| UNAUTHORIZED;

245 10 a| Sensitization of malignant breast epithelial cells to proton radiation by metallic nano-particles /c| by Nichole Libby.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2023.;

300 a| 1 online resource (95 pages) :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 12.77 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| Ph.D.c| East Carolina Universityd| 2023.;

500 a| Presented to the Faculty of the Department of Physics;

500 a| Advisor: Jefferson Shinpaugh;

500 a| Title from PDF t.p. (viewed September 24, 2024).;

520 3 a| Enhancing cancer treatment has garnered interest for more than 50 years. Enhancement has been considered in the evolution of treatment modalities as well as in the potential additives at the cellular level as radio-sensitizers. These enhancements all encompass the same goal: a method to spare healthy surrounding tissue while killing the cancerous tissue. In this work Polyethylene glycol (PEG)-coated gold nano-particles (GNPs) and super paramagnetic iron oxide nano-particles (SPIONs) were investigated as radio-sensitizers for malignant breast (MCF7) cells irradiated by energetic protons. The cells were cultured in the Department of Biology's Cell Culture Laboratory, where they were plated onto either custom 15-well plates or standard 96-well plates 48 hours prior to irradiation. The cells were then irradiated by 3 MeV protons in the East Carolina University Accelerator Laboratory in the Department of Physics. Twenty-four hours prior to irradiation the cells were treated with varying concentrations of the metallic nano-particles (NPs). Cell viability was determined using a color-metric assay (PrestoBlue), which was conducted prior to, as well as 24 hours post irradiation. Survival curves were determined based on the metabolic rate for each NP type and concentration post irradiation to observe potential sensitization effects. Sensitization effects were only observed for the PEG-coated GNPs at concentrations of 15 nM, 45 nM, and 68 nM. While each showed promise as radio sensitizers at the higher dose ranges (>6 Gy), the 45 nM concentration showed the greatest sensitization effects throughout the entire dose range (2-11 Gy).;

504 a| Includes bibliographical references.;

650 0 a| Breastx| Cancerx| Treatment.=| ^A990468;

650 0 a| Electronsx| Scattering.=| ^A38567;

650 0 a| Cancer cells.=| ^A97;

610 20 a| East Carolina University.=| ^A637090;

653 a| PEG-coated GNPs;

653 a| Protons;

653 a| MCF7;

700 1 a| Shinpaugh, Jefferson L.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Physics.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/13160;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6234509;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6234509-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/15/2023x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6234509-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/15/2023x| ETDz| HERESOURCE;

Sensitization of malignant breast epithelial cells to proton radiation by metallic nano-particles / by Nichole Libby.

Author/creator	Libby, Nichole author.
Other author	Shinpaugh, Jefferson L., degree supervisor.
Other author	East Carolina University. Department of Physics.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2023.
Description	1 online resource (95 pages) : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	East Carolina University. Breast--Cancer. --Treatment. Electrons--Scattering. Cancer cells.

Summary	Enhancing cancer treatment has garnered interest for more than 50 years. Enhancement has been considered in the evolution of treatment modalities as well as in the potential additives at the cellular level as radio-sensitizers. These enhancements all encompass the same goal: a method to spare healthy surrounding tissue while killing the cancerous tissue. In this work Polyethylene glycol (PEG)-coated gold nano-particles (GNPs) and super paramagnetic iron oxide nano-particles (SPIONs) were investigated as radio-sensitizers for malignant breast (MCF7) cells irradiated by energetic protons. The cells were cultured in the Department of Biology's Cell Culture Laboratory, where they were plated onto either custom 15-well plates or standard 96-well plates 48 hours prior to irradiation. The cells were then irradiated by 3 MeV protons in the East Carolina University Accelerator Laboratory in the Department of Physics. Twenty-four hours prior to irradiation the cells were treated with varying concentrations of the metallic nano-particles (NPs). Cell viability was determined using a color-metric assay (PrestoBlue), which was conducted prior to, as well as 24 hours post irradiation. Survival curves were determined based on the metabolic rate for each NP type and concentration post irradiation to observe potential sensitization effects. Sensitization effects were only observed for the PEG-coated GNPs at concentrations of 15 nM, 45 nM, and 68 nM. While each showed promise as radio sensitizers at the higher dose ranges (>6 Gy), the 45 nM concentration showed the greatest sensitization effects throughout the entire dose range (2-11 Gy).
General note	Presented to the Faculty of the Department of Physics
General note	Advisor: Jefferson Shinpaugh
General note	Title from PDF t.p. (viewed September 24, 2024).
Dissertation note	Ph.D. East Carolina University 2023.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Sensitization of malignant breast epithelial cells to proton radiation by metallic nano-particles / by Nichole Libby.

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