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035 a| (OCoLC)1381680553;

090 a| RC270.8;

049 a| EREE;

100 1 a| Halatek, David,e| author.?| UNAUTHORIZED;

245 10 a| Improving prostamide anti-cancer activity through derivatization and micellar delivery /c| by David Halatek.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2023.;

300 a| 1 online resource (59 pages) :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 4.639 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2023.;

500 a| Presented to the Faculty of the Department Chemistry;

500 a| Advisor: Colin Burns;

500 a| Title from PDF t.p. (viewed July 23, 2024).;

520 3 a| Colorectal cancer is the fourth most common cancer diagnosis per year as well as the fourth highest rate of death per year according to the Centers for Disease Control. Approximately 1/3rd of the diagnosed colorectal cancer cases per year will result in death. Prior research from our group has shown that the prostaglandin-ethanolamide 15-deoxy, [delta]12,14 prostamide J2 (15d-[delta]12,14-PMJ₂) is selectively toxic to murine melanoma cells (B16F10) and murine colorectal cells (CT-26) both in vitro and in vivo and significantly reduces tumor growth. Further, 15d-[delta]12,14-PMJ₂ induces cell death in primary patient melanoma cells and thus may be a promising therapeutic. As 15d-[delta]12,14-PMJ₂ can be made by the condensation of ethanolamine with 15-deoxy, [delta]12,14 prostaglandin J₂ (15d-[delta]12,14-PGJ₂), we sought to test the cytotoxicity of other prostamide derivatives to determine the structural features required for activity. Based on prior results in a study of related prodrugs, 15d-[delta]12,14-PMJ₂-Arvanil was selected as the top candidate for testing anti-cancer activity. After testing in both a human and murine cell line, it was determined that 15d-[delta]12,14-PMJ₂-Arvanil was not as cytotoxic as 15d-[delta]12,14-PMJ₂. It is possible that the bulkier functional group on the [alpha]-chain of the prostamide prevents transport into the cell the same way or to the same degree 15d-[delta]12,14-PMJ₂ enters. In an effort to test this hypothesis, and to develop an improved means for systemic delivery for this class of hydrophobic prostamides, engineered micelles composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] and D-[alpha]-tocopherol polyethylene glycol 1000 succinate were investigated as drug carriers.;

504 a| Includes bibliographical references.;

650 0 a| Cancerx| Treatment.=| ^A13628;

650 0 a| Colon (Anatomy)x| Cancerx| Treatment.=| ^A1195496;

650 0 a| Rectumx| Cancerx| Treatment.=| ^A1195502;

653 a| Cancer Therapeutics;

653 a| Small Molecule;

653 a| Prostamide;

653 a| Anandamide;

653 a| Prostaglandin;

653 a| J-series Prostaglandins;

653 a| J-series;

700 1 a| Burns, Colin Sanderson,e| degree supervisor.1| https://id.oclc.org/worldcat/entity/E39PCjvPtGJQyKYycqFrw6qBbm=| ^A1217829;

710 2 a| East Carolina University.b| Department of Chemistry.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/12851;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6143732;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6143732-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/9/2023x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6143732-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/9/2023x| ETDz| HERESOURCE;

Improving prostamide anti-cancer activity through derivatization and micellar delivery / by David Halatek.

Author/creator	Halatek, David author.
Other author	Burns, Colin Sanderson, degree supervisor.
Other author	East Carolina University. Department of Chemistry.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2023.
Description	1 online resource (59 pages) : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Cancer--Treatment. Colon (Anatomy)--Cancer. --Treatment. Rectum--Cancer. --Treatment.

Summary	Colorectal cancer is the fourth most common cancer diagnosis per year as well as the fourth highest rate of death per year according to the Centers for Disease Control. Approximately 1/3rd of the diagnosed colorectal cancer cases per year will result in death. Prior research from our group has shown that the prostaglandin-ethanolamide 15-deoxy, [delta]12,14 prostamide J2 (15d-[delta]12,14-PMJ₂) is selectively toxic to murine melanoma cells (B16F10) and murine colorectal cells (CT-26) both in vitro and in vivo and significantly reduces tumor growth. Further, 15d-[delta]12,14-PMJ₂ induces cell death in primary patient melanoma cells and thus may be a promising therapeutic. As 15d-[delta]12,14-PMJ₂ can be made by the condensation of ethanolamine with 15-deoxy, [delta]12,14 prostaglandin J₂ (15d-[delta]12,14-PGJ₂), we sought to test the cytotoxicity of other prostamide derivatives to determine the structural features required for activity. Based on prior results in a study of related prodrugs, 15d-[delta]12,14-PMJ₂-Arvanil was selected as the top candidate for testing anti-cancer activity. After testing in both a human and murine cell line, it was determined that 15d-[delta]12,14-PMJ₂-Arvanil was not as cytotoxic as 15d-[delta]12,14-PMJ₂. It is possible that the bulkier functional group on the [alpha]-chain of the prostamide prevents transport into the cell the same way or to the same degree 15d-[delta]12,14-PMJ₂ enters. In an effort to test this hypothesis, and to develop an improved means for systemic delivery for this class of hydrophobic prostamides, engineered micelles composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] and D-[alpha]-tocopherol polyethylene glycol 1000 succinate were investigated as drug carriers.
General note	Presented to the Faculty of the Department Chemistry
General note	Advisor: Colin Burns
General note	Title from PDF t.p. (viewed July 23, 2024).
Dissertation note	M.S. East Carolina University 2023.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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