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035 a| (Sirsi) o1381680408;

035 a| (OCoLC)1381680408;

049 a| EREE;

090 a| QP93.5;

100 1 a| Belcher, Heather A.,e| author.?| UNAUTHORIZED;

245 10 a| Evaluating the use of turbidimetry for studying fibrin structural properties /c| by Heather A. Belcher.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2023.;

300 a| 1 online resource (183 pages) :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 9.719 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| Ph.D.c| East Carolina Universityd| 2023.;

500 a| Presented to the Faculty of the Department of Physics;

500 a| Advisor: Nathan E. Hudson;

500 a| Title from PDF t.p. (viewed June 4, 2024).;

520 3 a| A fibrin fiber mesh forms the structural backbone of blood clots. Many pathological conditions result in fibrin gels with altered structural properties, so there is interest in developing rapid and accurate ways to characterize fibrin features. One method of determining the diameter and mass-length ratio of fibers is turbidimetry, which uses light scattering theory for randomly oriented, thin, cylindrical rods to estimate fibrin fiber structural properties from scattering data. There are several different approaches that use turbidimetry to solve for these parameters, each of which has different simplifications and assumptions of full light scattering theory. Although these different approaches are all commonly utilized, the validity of their approximations has not been investigated and the accuracy of the fitting parameters has not been tested against experimental data under a range of physiologically relevant conditions. Therefore, this research will evaluate the accuracy of the commonly utilized turbidimetric approaches by theoretically comparing them to full light scattering theory, and by comparing the acquired diameter values to those obtained experimentally using SEM and super-resolution imaging. The summation of this work will provide a framework for utilizing turbidimetry to study fibrin and pave a path for utilizing turbidimetry in a clinical setting.;

504 a| Includes bibliographical references.;

650 0 a| Fibrin.=| ^A835114;

650 0 a| Microscopy.=| ^A140;

650 0 a| Hemostasis.=| ^A4809;

650 0 a| Lightx| Scattering.=| ^A32302;

653 a| turbidimetry;

653 a| fiber diameter;

653 a| fluorescent microscopy;

653 a| scanning electron microscopy;

700 1 a| Hudson, Nathan E.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Physics.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/12817;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6143687;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6143687-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/9/2023x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6143687-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/9/2023x| ETDz| HERESOURCE;

Evaluating the use of turbidimetry for studying fibrin structural properties / by Heather A. Belcher.

Author/creator	Belcher, Heather A. author.
Other author	Hudson, Nathan E., degree supervisor.
Other author	East Carolina University. Department of Physics.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2023.
Description	1 online resource (183 pages) : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Fibrin. Microscopy. Hemostasis. Light--Scattering.

Summary	A fibrin fiber mesh forms the structural backbone of blood clots. Many pathological conditions result in fibrin gels with altered structural properties, so there is interest in developing rapid and accurate ways to characterize fibrin features. One method of determining the diameter and mass-length ratio of fibers is turbidimetry, which uses light scattering theory for randomly oriented, thin, cylindrical rods to estimate fibrin fiber structural properties from scattering data. There are several different approaches that use turbidimetry to solve for these parameters, each of which has different simplifications and assumptions of full light scattering theory. Although these different approaches are all commonly utilized, the validity of their approximations has not been investigated and the accuracy of the fitting parameters has not been tested against experimental data under a range of physiologically relevant conditions. Therefore, this research will evaluate the accuracy of the commonly utilized turbidimetric approaches by theoretically comparing them to full light scattering theory, and by comparing the acquired diameter values to those obtained experimentally using SEM and super-resolution imaging. The summation of this work will provide a framework for utilizing turbidimetry to study fibrin and pave a path for utilizing turbidimetry in a clinical setting.
General note	Presented to the Faculty of the Department of Physics
General note	Advisor: Nathan E. Hudson
General note	Title from PDF t.p. (viewed June 4, 2024).
Dissertation note	Ph.D. East Carolina University 2023.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Evaluating the use of turbidimetry for studying fibrin structural properties / by Heather A. Belcher.

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