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035 a| (Sirsi) o1332540313;

035 a| (OCoLC)1332540313;

049 a| EREE;

090 a| QL638.C94;

100 1 a| Cedrone, Alexandria,e| author.?| UNAUTHORIZED;

245 10 a| Development of an optogenetic tool for targeted sensory hair cell ablation in zebrafish /c| by Alexandria Cedrone.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2022.;

300 a| 1 online resource (85 pages) :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text file;

347 b| PDF;

347 c| 17.51 MB;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2022;

500 a| Presented to the Faculty of the Department of Biology.;

500 a| Advisor: Elizabeth T. Ables;

500 a| Title from PDF t.p. (viewed June 22, 2023).;

520 3 a| Mechanosensory hair cells convert mechanical information into neuronal signals. Mutations in the genes required for hair cell function cause deafness and lead to the degeneration of sensory hair cells. Mammals are unable to regenerate these cells, leading to the permanent loss of hair cells. Research efforts are being made to stimulate hair cell regeneration in mammals and to repair genetic mutations via gene therapy to restore hearing. However, if sensory-deficient hair cells are unable to be regenerated, the effectiveness of gene therapy will be limited. To date, there are no studies examining whether mutant hair cells are able to regenerate after cell death. Other vertebrates, such as amphibians, birds, reptiles and fish, possess the ability to regenerate mechanosensory hair cells after cell death. Thus, it is of great value to investigate the genetic factors affecting the regeneration of the sensory hair cells in these species as a step towards initiating this ability in humans. This experiment uses the zebrafish model system to investigate the ability of sensory- deficient hair cells to resist ototoxic insults, and the proliferative and regenerative properties of the lateral line organ. To look at the regeneration capabilities of MET mutants, ablation methods were used to target sensory hair cells using the OptoBax system in zebrafish.;

504 a| Includes bibliographical references.;

650 0 a| Zebra daniox| Physiology.=| ^A584582;

650 0 a| Hair cellsx| Regeneration.=| ^A1458733;

653 a| Zebrafish;

653 a| OptoBAX;

653 a| Otic;

653 a| Ototoxin;

653 a| Sensory hair cell;

653 a| Mechanosensory hair cell;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

700 1 a| Ables, Elizabeth Tweedie,e| degree supervisor.=| ^A1314283;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 u| http://hdl.handle.net/10342/10701z| Access via ScholarShip;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5831667;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5831667-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/23/2022x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5831667-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/23/2022x| ETDz| HERESOURCE;

Development of an optogenetic tool for targeted sensory hair cell ablation in zebrafish / by Alexandria Cedrone.

Author/creator	Cedrone, Alexandria author.
Other author	Ables, Elizabeth Tweedie, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2022.
Description	1 online resource (85 pages) : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	Zebra danio--Physiology. Hair cells--Regeneration.

Summary	Mechanosensory hair cells convert mechanical information into neuronal signals. Mutations in the genes required for hair cell function cause deafness and lead to the degeneration of sensory hair cells. Mammals are unable to regenerate these cells, leading to the permanent loss of hair cells. Research efforts are being made to stimulate hair cell regeneration in mammals and to repair genetic mutations via gene therapy to restore hearing. However, if sensory-deficient hair cells are unable to be regenerated, the effectiveness of gene therapy will be limited. To date, there are no studies examining whether mutant hair cells are able to regenerate after cell death. Other vertebrates, such as amphibians, birds, reptiles and fish, possess the ability to regenerate mechanosensory hair cells after cell death. Thus, it is of great value to investigate the genetic factors affecting the regeneration of the sensory hair cells in these species as a step towards initiating this ability in humans. This experiment uses the zebrafish model system to investigate the ability of sensory- deficient hair cells to resist ototoxic insults, and the proliferative and regenerative properties of the lateral line organ. To look at the regeneration capabilities of MET mutants, ablation methods were used to target sensory hair cells using the OptoBax system in zebrafish.
General note	Presented to the Faculty of the Department of Biology.
General note	Advisor: Elizabeth T. Ables
General note	Title from PDF t.p. (viewed June 22, 2023).
Dissertation note	M.S. East Carolina University 2022
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.
Genre/form	Academic theses.
Genre/form	Academic theses.

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Development of an optogenetic tool for targeted sensory hair cell ablation in zebrafish / by Alexandria Cedrone.

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