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001 ocm33233903;

003 OCoLC;

005 20251021154323.0;

008 951003s1994 xx af bm 000 0 eng d;

035 a| (Sirsi) o33233903;

035 a| (OCoLC)33233903;

049 a| EREE;

050 4 a| QL668.C28b| N48 1994;

100 1 a| Nguyen, Ngan-Ha Thai,e| author.?| UNAUTHORIZED;

245 14 a| The effects of 1,1,3 tricyano-2-amino-1-propene (TRIAP) and retinoic acid on limb regeneration in the red-spotted newt, Notophthalmus viridescens /c| by Ngan-Ha Thai Nguyen.;

264 0 c| 1994.;

300 a| 82 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1994;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Charles A. Singhas;

520 3 a| The present study partially characterizes the terminal carbohydrate moieties of glycoconjugates during limb regeneration using lectin histochemistry, and examines the distribution of "neural elements" using Bodian histochemistry in regenerating limbs treated with Triap and/or retinoic acid (RA) at day 46 post amputation. A panel of eleven horseradish peroxidase conjugate (HRP) lectins were used to probe epidermal glycoconjugates for specific carbohydrate moieties. The eleven lectins utilized are LTA, UEA-I, DBA, GSAI-B4, GSA-II, Con A, Scon A, WGA, PN°, and ECA. Most regenerating tissues exhibited affinity for seven specific lectins by retaining their binding characteristics at the end of the 46-day experimental period. Tissues treated with Triap and/or RA revealed similar terminal carbohydrate moieties of glycoconjugates and the distribution of "neural elements". Extracellular matrix of the regenerating limbs contributed in directing regeneration. These findings demonstrate that Triap and/or RA brought about integumental changes during limb regeneration in the red-spotted newt.;

504 a| Includes bibliographical references (leaves 73-77).;

650 0 a| Notophthalmus viridescensx| Physiology.=| ^A348045;

650 0 a| Extremities (Anatomy)x| Regeneration.=| ^A158113;

650 0 a| Tretinoin.=| ^A368381;

650 2 a| Tretinoin.0| (DNLM)D014212=| ^A1289074;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Extremities (Anatomy)x| Regeneration.2| fast0| (OCoLC)fst00919089;

650 7 a| Notophthalmus viridescensx| Physiology.2| fast0| (OCoLC)fst01039640;

650 7 a| Tretinoin.2| fast0| (OCoLC)fst01156231;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Singhas, Charles A.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12651;

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The effects of 1,1,3 tricyano-2-amino-1-propene (TRIAP) and retinoic acid on limb regeneration in the red-spotted newt, Notophthalmus viridescens / by Ngan-Ha Thai Nguyen.

Author/creator	Nguyen, Ngan-Ha Thai author.
Other author	Singhas, Charles A., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1994.
Description	82 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Notophthalmus viridescens--Physiology. Extremities (Anatomy)--Regeneration. Tretinoin. Tretinoin.

Summary	The present study partially characterizes the terminal carbohydrate moieties of glycoconjugates during limb regeneration using lectin histochemistry, and examines the distribution of "neural elements" using Bodian histochemistry in regenerating limbs treated with Triap and/or retinoic acid (RA) at day 46 post amputation. A panel of eleven horseradish peroxidase conjugate (HRP) lectins were used to probe epidermal glycoconjugates for specific carbohydrate moieties. The eleven lectins utilized are LTA, UEA-I, DBA, GSAI-B4, GSA-II, Con A, Scon A, WGA, PN°, and ECA. Most regenerating tissues exhibited affinity for seven specific lectins by retaining their binding characteristics at the end of the 46-day experimental period. Tissues treated with Triap and/or RA revealed similar terminal carbohydrate moieties of glycoconjugates and the distribution of "neural elements". Extracellular matrix of the regenerating limbs contributed in directing regeneration. These findings demonstrate that Triap and/or RA brought about integumental changes during limb regeneration in the red-spotted newt.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Charles A. Singhas
Dissertation note	M.S. East Carolina University 1994
Bibliography note	Includes bibliographical references (leaves 73-77).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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The effects of 1,1,3 tricyano-2-amino-1-propene (TRIAP) and retinoic acid on limb regeneration in the red-spotted newt, Notophthalmus viridescens / by Ngan-Ha Thai Nguyen.

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