Polyamines in human intestinal anaerobic bacteria / by William D. Proctor.
| Author/creator | Proctor, William D. author. |
| Other author | Stellwag, Edmund J. (Edmund Johannes), 1951- degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1994. |
| Description | 84 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Subject | Polyamines are a group of aliphatic polycationic compounds that are found ubiquitously in both procaryotic and eucaryotic organisms and are necessary for normal cell growth. Recent evidence indicates that polyamines produced by anaerobic bacteria in the gastrointestinal (GI) tract of vertebrates influence host GI epithelial cell growth. Six numerically dominant members of the bacterial flora of the human GI tract; Bacteroides distasonis, B. thetaiotaomicron, B. vulgatus, Bifidobacterium adolescentis Euhacterium aerofaciens and Peptostreptococcus productus were cultured in the presence and absence of glucose. Cell associated and extracellular concentrations of putrescine, cadaverino, spermidine, spermine and the acetylated derivatives of these compounds were determined by reversed phase HPLC chromatograpy. Spermidine and spermine were the only cell-associated polyamines detected in the three Bacteroides spp., E. aerofaciens and B. adolescentis. Spermidine and spermine were absent in E. aerofaciens and B. adolescentis. P. productus contained cell-associated spermidine, spermine and N₁-acetylputrescine. Growth-phase and glucose-dependent differences in cell-associated polyamine concentrations were observed in most species. Glucose may repress transport of spermidine in E. aerofaciens and B. adolescentis. Calculation of total stationary phase polyamine content indicates that substantial quantities of exogenous polyamines are transported into the cells and metabolized. Indirect evidence indicates that putrescine may be synthesized de novo by P. productus and converted to acetylputrescine. Four additional species also produce and excrete cadaverine and acetylspermine. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Edmund J. Stellwag |
| Dissertation note | M.S. East Carolina University 1994 |
| Bibliography note | Includes bibliographical references (leaves 79-84). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | ✔ Available |