Tools

LEADER 03368cam a2200517Ii 4500

001 on1268150184;

003 OCoLC;

005 20220428041122.0;

006 m o d ;

007 cr unu||||||||;

008 210914s2021 ncua obm 000 0 eng d;

035 a| (Sirsi) o1268150184;

035 a| (OCoLC)1268150184;

049 a| EREE;

090 a| QL537.D76;

100 1 a| Beachum, Allison N.,e| author.?| UNAUTHORIZED;

245 14 a| The small GTPase Ran and [beta]-importins Tnpo-SR and Cadmus promote ovarian cyst formation in drosophila /c| by Allison N. Beachum.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2021.;

300 a| 61 pages :b| color illustrations;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 2.841 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2021.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Elizabeth T. Ables;

500 a| Title from PDF t.p. (viewed April 26, 2022).;

520 3 a| Germ cells follow a precise and coordinated molecular timeline to produce a viable oocyte. While undergoing mitotic expansion via incomplete cytokinesis, cysts of undifferentiated cells form and remain interconnected prior to meiotic initiation, through mechanisms that are not well-defined. In somatic cells, Ras-related nuclear protein (Ran) spatiotemporally regulates mitotic spindle assembly, cleavage furrow formation and abscission. Here, we identify Ran and [beta]-importins as critical regulators of cyst development in the Drosophila ovary. Depletion of Ran or the [beta]-importins Tnpo-SR and Cadmus results in egg chambers with variable numbers of germ cells, suggesting abnormal cyst development and cyst fragmentation, and consequently disrupts oocyte selection. We demonstrate that Ran, Tnpo-SR, and Cadmus regulate key cellular processes during cyst formation, including cell cycle dynamics, fusome biogenesis, and ring canal stability, all independently of mitotic spindle assembly. Further, Tnpo-SR and Cadmus control cyclin accumulation and suppress cytokinesis, suggesting that [beta]-importins sequester protein cargos that normally promote the mitotic-to-meiotic transition. Our data demonstrates that Ran and [beta]-importins are critical for the cell fate decisions of germ cells, a role that is likely conserved in other organisms.;

504 a| Includes bibliographical references.;

650 0 a| Drosophilax| Genetic aspects.=| ^A54199;

650 0 a| Oogenesis.=| ^A241829;

653 a| stem cells;

653 a| karyopherin;

653 a| oocyte;

700 1 a| Ables, Elizabeth Tweedie,e| degree supervisor.=| ^A1314283;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/9408;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5668776;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5668776-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/14/2021x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5668776-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/14/2021x| ETDz| HERESOURCE;

The small GTPase Ran and [beta]-importins Tnpo-SR and Cadmus promote ovarian cyst formation in drosophila / by Allison N. Beachum.

Author/creator	Beachum, Allison N. author.
Other author	Ables, Elizabeth Tweedie, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2021.
Description	61 pages : color illustrations
Supplemental Content	Access via ScholarShip
Subjects	Drosophila--Genetic aspects. Oogenesis.

Summary	Germ cells follow a precise and coordinated molecular timeline to produce a viable oocyte. While undergoing mitotic expansion via incomplete cytokinesis, cysts of undifferentiated cells form and remain interconnected prior to meiotic initiation, through mechanisms that are not well-defined. In somatic cells, Ras-related nuclear protein (Ran) spatiotemporally regulates mitotic spindle assembly, cleavage furrow formation and abscission. Here, we identify Ran and [beta]-importins as critical regulators of cyst development in the Drosophila ovary. Depletion of Ran or the [beta]-importins Tnpo-SR and Cadmus results in egg chambers with variable numbers of germ cells, suggesting abnormal cyst development and cyst fragmentation, and consequently disrupts oocyte selection. We demonstrate that Ran, Tnpo-SR, and Cadmus regulate key cellular processes during cyst formation, including cell cycle dynamics, fusome biogenesis, and ring canal stability, all independently of mitotic spindle assembly. Further, Tnpo-SR and Cadmus control cyclin accumulation and suppress cytokinesis, suggesting that [beta]-importins sequester protein cargos that normally promote the mitotic-to-meiotic transition. Our data demonstrates that Ran and [beta]-importins are critical for the cell fate decisions of germ cells, a role that is likely conserved in other organisms.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Elizabeth T. Ables
General note	Title from PDF t.p. (viewed April 26, 2022).
Dissertation note	M.S. East Carolina University 2021.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Availability

Library	Location	Call Number	Status	Item Actions
	Electronic Resources	Access Content Online	✔ Available

The small GTPase Ran and [beta]-importins Tnpo-SR and Cadmus promote ovarian cyst formation in drosophila / by Allison N. Beachum.

Click here for more information about this title

Availability