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003 OCoLC;

005 20210812123036.0;

006 m o d ;

007 cr unu||||||||;

008 210114s2020 ncua obm 000 0 eng d;

035 a| (Sirsi) o1231501004;

035 a| (OCoLC)1231501004;

049 a| EREE;

090 a| RA1231.N44;

100 1 a| Turner, Ryne,e| author.?| UNAUTHORIZED;

245 10 a| Identifying compensatory genomic changes due to nickel (Ni²⁺) exposure :b| electrochemistry and quantifying DNA damage /c| Ryne Turner.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2020.;

300 a| 60 pages :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 2.905 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2020.;

500 a| Presented to the faculty of the Department of Chemistry;

500 a| Advisor: David J. Rudel;

500 a| Advisor: Eli G. Hvastkovs;

500 a| Title from PDF t.p. (viewed August 10, 2021).;

520 3 a| Nickel is a toxic heavy metal that has been shown to cause adverse healthconditions in organisms. DNA damage has been observed in nematodes in response to nickel exposure. Nematodes originating from environments with high Ni concentrations have shown an increased resistance to Ni exposure. Recombinant inbred lines (RIAILS) have been created using a cross between a nematode originating from a Ni rich environment, with a nematode from an environment with low levels of Ni. In this study, RIAILs were grown on Ni treated plates and Ni absent plates. DNA from the different cultures were extracted and studied using electrochemistry and mass spectrometry. This study shows that RIAILs exhibit a varied response to Ni exposure. RIAILs with lower DNA damage may have advantageous genes reducing the amount of DNA damage in the organism in response to nickel exposure. Contrarily, nematodes with higher DNA damage may have inherited genes that make them more susceptible to Ni exposure. The varying response in DNA damage between RIAILs suggests there are multiple genes involved with Ni exposure. Future studies involving the outliers can lead to a better understanding of the mechanism(s) by which Ni causes DNA damage.;

504 a| Includes bibliographical references.;

650 0 a| Nickelx| Toxicology.=| ^A38760;

650 0 a| Nematodesx| Anatomy.=| ^A278460;

650 0 a| DNA damage.=| ^A273441;

653 a| Toxicity;

700 1 a| Rudel, David,e| degree supervisor.=| ^A1157217;

700 1 a| Hvastkovs, Eli G.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Chemistry.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/8838;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5547878;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5547878-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 1/14/2021x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5547878-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 1/14/2021x| ETDz| HERESOURCE;

Identifying compensatory genomic changes due to nickel (Ni²⁺) exposure : electrochemistry and quantifying DNA damage / Ryne Turner.

Author/creator	Turner, Ryne author.
Other author	Rudel, David, degree supervisor.
Other author	Hvastkovs, Eli G., degree supervisor.
Other author	East Carolina University. Department of Chemistry.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2020.
Description	60 pages : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	Nickel--Toxicology. Nematodes--Anatomy. DNA damage.

Summary	Nickel is a toxic heavy metal that has been shown to cause adverse healthconditions in organisms. DNA damage has been observed in nematodes in response to nickel exposure. Nematodes originating from environments with high Ni concentrations have shown an increased resistance to Ni exposure. Recombinant inbred lines (RIAILS) have been created using a cross between a nematode originating from a Ni rich environment, with a nematode from an environment with low levels of Ni. In this study, RIAILs were grown on Ni treated plates and Ni absent plates. DNA from the different cultures were extracted and studied using electrochemistry and mass spectrometry. This study shows that RIAILs exhibit a varied response to Ni exposure. RIAILs with lower DNA damage may have advantageous genes reducing the amount of DNA damage in the organism in response to nickel exposure. Contrarily, nematodes with higher DNA damage may have inherited genes that make them more susceptible to Ni exposure. The varying response in DNA damage between RIAILs suggests there are multiple genes involved with Ni exposure. Future studies involving the outliers can lead to a better understanding of the mechanism(s) by which Ni causes DNA damage.
General note	Presented to the faculty of the Department of Chemistry
General note	Advisor: David J. Rudel
General note	Advisor: Eli G. Hvastkovs
General note	Title from PDF t.p. (viewed August 10, 2021).
Dissertation note	M.S. East Carolina University 2020.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Identifying compensatory genomic changes due to nickel (Ni²⁺) exposure : electrochemistry and quantifying DNA damage / Ryne Turner.

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