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LEADER 03632ctm a2200529 i 4500

001 ocm30651405;

003 OCoLC;

005 20260420152328.0;

008 940623s1993 xx bm 000 0 eng d;

035 a| (Sirsi) o30651405;

035 a| (OCoLC)30651405;

049 a| EREE;

050 4 a| QL638.P358b| W34 1993;

100 1 a| Walker, Robert B.,e| author.=| ^A1211769;

245 10 a| Intraspecies sequence variability of the major histocompatibility complex class II [beta] chain in striped bass, Morone saxatilis /c| by Robert B. Walker.;

264 0 c| 1993.;

300 a| 71 leaves ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1993;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Thomas J. McConnell;

520 3 a| This study reports the initial isolation and characterization of Major histocompatibility complex {Mhc) class II (beta) genes in the teleost, Morone saxatilis. Polymerase chain reaction (PCR) and dideoxy sequencing techniques were employed to amplify and characterize seven subclones (Mosa-C-1, Mosa-C-2, Mosa-C22, Mosa-R41, Mosa-S-1, Mosa-S-2, and Mosa-S-3) encoding the entire mature Mhc class II (beta) chain. Striped bass from three different locations (Roanoke River, Chesapeake Bay, and Santee-Cooper Reservoir) were used in this study. Extensive polymorphism was found in the (beta)1 domain encoding region of the cDNA, which corresponds with the functional peptide binding region (PBR) of known Mhc class II molecules (Figure 4). Variable amino acid residues in the (beta)1 domains from the striped bass clones correspond with polymorphic sites of (beta)1 domains from other teleosts and higher vertebrates. Other regions of the striped bass Mhc class II translated proteins correlated well with those of known vertebrate Mhc class II proteins. Seventy-one percent of the variability within the presumed PBR of the striped bass Mhc class II (beta) chain gene was found to correspond with that of the PBR of a human Mhc class II (beta) chain gene (Brown et al. 1993).;

504 a| Includes bibliographical references (leaves 59-63).;

650 0 a| Striped bass.=| ^A69980;

650 0 a| Fishesx| Variation.=| ^A348043;

650 0 a| Fishesx| Immunology.=| ^A285730;

650 7 a| Fishesx| Immunology.2| fast0| (OCoLC)fst00926518;

650 7 a| Fishesx| Variation.2| fast0| (OCoLC)fst00926634;

650 7 a| Striped bass.2| fast0| (OCoLC)fst01135489;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| McConnell, Thomas J.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12899;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 549725;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 958693d| 1/22/2002l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 1/22/2002x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 549725-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 3/16/2026x| ETDz| JERESOURCE;

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Intraspecies sequence variability of the major histocompatibility complex class II [beta] chain in striped bass, Morone saxatilis / by Robert B. Walker.

Author/creator	Walker, Robert B. author.
Other author	McConnell, Thomas J., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1993.
Description	71 leaves ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Striped bass. Fishes--Variation. Fishes--Immunology.

Summary	This study reports the initial isolation and characterization of Major histocompatibility complex {Mhc) class II (beta) genes in the teleost, Morone saxatilis. Polymerase chain reaction (PCR) and dideoxy sequencing techniques were employed to amplify and characterize seven subclones (Mosa-C-1, Mosa-C-2, Mosa-C22, Mosa-R41, Mosa-S-1, Mosa-S-2, and Mosa-S-3) encoding the entire mature Mhc class II (beta) chain. Striped bass from three different locations (Roanoke River, Chesapeake Bay, and Santee-Cooper Reservoir) were used in this study. Extensive polymorphism was found in the (beta)1 domain encoding region of the cDNA, which corresponds with the functional peptide binding region (PBR) of known Mhc class II molecules (Figure 4). Variable amino acid residues in the (beta)1 domains from the striped bass clones correspond with polymorphic sites of (beta)1 domains from other teleosts and higher vertebrates. Other regions of the striped bass Mhc class II translated proteins correlated well with those of known vertebrate Mhc class II proteins. Seventy-one percent of the variability within the presumed PBR of the striped bass Mhc class II (beta) chain gene was found to correspond with that of the PBR of a human Mhc class II (beta) chain gene (Brown et al. 1993).
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Thomas J. McConnell
Dissertation note	M.S. East Carolina University 1993
Bibliography note	Includes bibliographical references (leaves 59-63).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Intraspecies sequence variability of the major histocompatibility complex class II [beta] chain in striped bass, Morone saxatilis / by Robert B. Walker.

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