Tools

LEADER 03455cam a2200505Ii 4500

001 on1056953828;

003 OCoLC;

005 20190116100151.0;

006 m o d ;

007 cr unu||||||||;

008 181015s2018 ncua obm 000 0 eng d;

035 a| (Sirsi) o1056953828;

035 a| (OCoLC)1056953828;

049 a| EREE;

090 a| TP382;

100 1 a| Angermeier, Tori Marena,e| author.?| UNAUTHORIZED;

245 10 a| Using fluorescence and infrared spectroscopy to analyze the interaction of disaccharides with lipid bilayers /c| by Tori Marena Angermeier.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2018.;

300 a| 61 pages :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 1.881 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2018.;

500 a| Presented to the faculty of the Department of Chemistry;

500 a| Advisor: Anthony Kennedy;

500 a| Title from PDF t.p. (viewed December 5, 2018).;

520 3 a| It is well known that sugars, such as trehalose, dehydrate lipid membranes based upon the Water Replacement Hypothesis. Here, we have investigated the interaction between different sugars (sucralose, sucrose, and trehalose) and model phospholipid membrane 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) using fluorescence spectroscopy with the fluorescent probe Laurdan and infrared spectroscopy. For the fluorescence measurements, the addition of sugar molecules to the bilayer resulted in a decrease in the Generalized Polarization (GP) values, corresponding to an increase in polarity in the environment within the bilayer around the probe. A decrease in GP values of Laurdan also suggest the possibility of coexisting phases within the bilayer. Infrared spectroscopy was used to investigate the dynamics of the hydrophilic and hydrophobic regions of the membrane as the changes of the asymmetric phosphate group and fatty acid chain vibrational bands were monitored when introduced to sucrose and sucralose. These tests revealed that sucrose had no significant impact on the membrane in either region of the bilayer, while sucralose showed a significant impact on both regions of the bilayer. Overall, we have monitored the interaction of different sugar molecules with model DPPC membranes through fluorescence and infrared spectroscopy measurements to explore the polarity of the bilayer and the possible dynamic effects of sugars have on lipid membranes.;

504 a| Includes bibliographical references.;

650 0 a| Sugarx| Analysis.=| ^A652303;

650 0 a| Disaccharides.=| ^A1304687;

650 0 a| Fluorescence spectroscopy.=| ^A3307;

653 a| lipid;

700 1 a| Kennedy, Anthony,e| degree supervisor.=| ^A1267910;

710 2 a| East Carolina University.b| Department of Chemistry.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/6929;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 4931009;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4931009-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 10/15/2018x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4931009-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 10/15/2018x| ETDz| HERESOURCE;

Using fluorescence and infrared spectroscopy to analyze the interaction of disaccharides with lipid bilayers / by Tori Marena Angermeier.

Author/creator	Angermeier, Tori Marena author.
Other author	Kennedy, Anthony, degree supervisor.
Other author	East Carolina University. Department of Chemistry.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2018.
Description	61 pages : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Sugar--Analysis. Disaccharides. Fluorescence spectroscopy.

Summary	It is well known that sugars, such as trehalose, dehydrate lipid membranes based upon the Water Replacement Hypothesis. Here, we have investigated the interaction between different sugars (sucralose, sucrose, and trehalose) and model phospholipid membrane 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) using fluorescence spectroscopy with the fluorescent probe Laurdan and infrared spectroscopy. For the fluorescence measurements, the addition of sugar molecules to the bilayer resulted in a decrease in the Generalized Polarization (GP) values, corresponding to an increase in polarity in the environment within the bilayer around the probe. A decrease in GP values of Laurdan also suggest the possibility of coexisting phases within the bilayer. Infrared spectroscopy was used to investigate the dynamics of the hydrophilic and hydrophobic regions of the membrane as the changes of the asymmetric phosphate group and fatty acid chain vibrational bands were monitored when introduced to sucrose and sucralose. These tests revealed that sucrose had no significant impact on the membrane in either region of the bilayer, while sucralose showed a significant impact on both regions of the bilayer. Overall, we have monitored the interaction of different sugar molecules with model DPPC membranes through fluorescence and infrared spectroscopy measurements to explore the polarity of the bilayer and the possible dynamic effects of sugars have on lipid membranes.
General note	Presented to the faculty of the Department of Chemistry
General note	Advisor: Anthony Kennedy
General note	Title from PDF t.p. (viewed December 5, 2018).
Dissertation note	M.S. East Carolina University 2018.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Availability

Library	Location	Call Number	Status	Item Actions
	Electronic Resources	Access Content Online	✔ Available

Using fluorescence and infrared spectroscopy to analyze the interaction of disaccharides with lipid bilayers / by Tori Marena Angermeier.

Click here for more information about this title

Availability