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001 on1021186371;

003 OCoLC;

005 20180314112601.0;

006 m o d ;

007 cr unu||||||||;

008 180201s2017 ncua obm 000 0 eng d;

035 a| (Sirsi) o1021186371;

035 a| (OCoLC)1021186371;

049 a| EREE;

090 a| QL458.42.T48;

100 1 a| Hasan, Zarif,e| author.?| UNAUTHORIZED;

245 14 a| The effect of diet on venom gene expression in Tetragnatha versicolor /c| by Zarif Hasan.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2017.;

300 a| 69 pages :b| color illustrations;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 1.025 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2017.;

500 a| Presented to the faculty of the Department of Biology;

500 a| Advisor: Michael S. Brewer;

500 a| Title from PDF t.p. (viewed February 16, 2018).;

520 3 a| Tetragnatha versicolor is an orb weaving spider that can be found throughout North America from Alaska to Nicaragua. This along with the fact the T. versicolor is a habitat generalist means that the species encounters a diverse array of insect prey species throughout its range. The purpose of this study is to determine if two populations of T. versicolor that feed on different insect species also differentially express venom genes. First, I determined whether two populations of T. versicolor were eating different prey species by measuring the [delta]15N values of T. versicolor from each site. The results of this analysis were that the [delta]15N values for the two populations of T. versicolor were significantly different. This suggests that the two populations are at different trophic levels which indicates that the two populations of T. versicolor are eating different insect species. Next, I used the RNA isolated from the venom glands of three males and three females from each of the two populations to construct a venom gland transcriptome which I used to perform a differential expression analysis comparing the males of each population, the females of each population, and the males and females within each population. The results of this analysis showed that when comparing the females of each population, they were differentially expressing genes that are likely to be venom related. Also, when comparing the males to females, the males of each population were differentially expressing potential venom related genes despite the fact that their isotopic signatures were not significantly different from those of the females in the same population.;

504 a| Includes bibliographical references.;

650 0 a| Tetragnathidaex| Venomx| Toxicology.=| ^A1356626;

650 0 a| Sexual dimorphism (Animals)=| ^A9115;

653 a| evolution;

700 1 a| Brewer, Michael S.,e| degree supervisor.=| ^A1354030;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/6461;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 4814934;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4814934-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 2/1/2018x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4814934-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 2/1/2018x| ETDz| HERESOURCE;

The effect of diet on venom gene expression in Tetragnatha versicolor / by Zarif Hasan.

Author/creator	Hasan, Zarif author.
Other author	Brewer, Michael S., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2017.
Description	69 pages : color illustrations
Supplemental Content	Access via ScholarShip
Subjects	Tetragnathidae--Venom. --Toxicology. Sexual dimorphism (Animals)

Summary	Tetragnatha versicolor is an orb weaving spider that can be found throughout North America from Alaska to Nicaragua. This along with the fact the T. versicolor is a habitat generalist means that the species encounters a diverse array of insect prey species throughout its range. The purpose of this study is to determine if two populations of T. versicolor that feed on different insect species also differentially express venom genes. First, I determined whether two populations of T. versicolor were eating different prey species by measuring the [delta]15N values of T. versicolor from each site. The results of this analysis were that the [delta]15N values for the two populations of T. versicolor were significantly different. This suggests that the two populations are at different trophic levels which indicates that the two populations of T. versicolor are eating different insect species. Next, I used the RNA isolated from the venom glands of three males and three females from each of the two populations to construct a venom gland transcriptome which I used to perform a differential expression analysis comparing the males of each population, the females of each population, and the males and females within each population. The results of this analysis showed that when comparing the females of each population, they were differentially expressing genes that are likely to be venom related. Also, when comparing the males to females, the males of each population were differentially expressing potential venom related genes despite the fact that their isotopic signatures were not significantly different from those of the females in the same population.
General note	Presented to the faculty of the Department of Biology
General note	Advisor: Michael S. Brewer
General note	Title from PDF t.p. (viewed February 16, 2018).
Dissertation note	M.S. East Carolina University 2017.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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The effect of diet on venom gene expression in Tetragnatha versicolor / by Zarif Hasan.

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