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001 on1001281715;

003 OCoLC;

005 20180110101631.0;

006 m o d ;

007 cr unu||||||||;

008 170816s2017 ncua obm 000 0 eng d;

035 a| (Sirsi) o1001281715;

035 a| (OCoLC)1001281715;

049 a| EREE;

090 a| QP571.7;

100 1 a| Berghout, Hanna E.,e| author.?| UNAUTHORIZED;

245 10 a| Drosophila ovarian stem cell establishment is regulated by nuclear hormone receptor ftz-f1 /c| by Hanna E. Berghout.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2017.;

300 a| 93 pages :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 3.307 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2017;

500 a| Presented to the faculty of the Department of Biology;

500 a| Advisor: Elizabeth Ables;

500 a| Title from PDF t.p. (viewed January 2, 2018).;

520 3 a| Stem cells are a fundamental underpinning of tissue biology. Loss of the self-renewing function of stem cells leads to conditions such as infertility and tissue wasting. Stem cells integrate a variety of signals to maintain their fate and proliferative capacity. Although intrinsic and local cues are well studied, less is known about how extrinsic signals, such as hormones, affect stem cell fate and function. The highly characterized Drosophila melanogaster steroid hormone ecdysone regulates germline stem cell (GSC) proliferation and self-renewal, as well as oogenesis and metamorphosis. Though many genes, including nuclear hormone receptor ftz transcription factor 1 (ftz-f1), are thought to be targets of ecdysone signaling, it is unclear how these targets impact GSC fate and function. To explore the role of ftz-f1 in ovarian stem cells, we used the UAS-GAL4 system and RNA interference (RNAi) to reduce ftz-f1 function specifically in germ cells or surrounding somatic cells. We demonstrate that ftz-f1 is intrinsically required for the establishment of the proper number of GSCs during development. Reduced ftz-f1 function in germ cells leads to a significant decrease in average number of GSCs. During larval stages, ftz-f1 depleted ovaries contain a number of PGCs located significantly further away from the terminal filament stacks. Our results also suggest that ftz-f1 is required in ovarian somatic cells during development for proper movement of germ cells out of the germarium in adult stages. Taken together, we suggest ftz-f1 function during juvenile stages is critical for the establishment of GSCs and development of their progeny.;

504 a| Includes bibliographical references.;

650 0 a| Hormone receptors.=| ^A8335;

650 0 a| Drosophila melanogaster.=| ^A86209;

650 0 a| Stem cells.=| ^A390101;

650 0 a| Ecdysone.=| ^A122555;

653 a| establishment;

653 a| primordial germ cells;

653 a| development;

653 a| niche;

700 1 a| Ables, Elizabeth Tweedie,e| degree supervisor.=| ^A1314283;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/6383;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 4733398;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4733398-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 8/16/2017x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4733398-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 8/16/2017x| ETDz| HERESOURCE;

Drosophila ovarian stem cell establishment is regulated by nuclear hormone receptor ftz-f1 / by Hanna E. Berghout.

Author/creator	Berghout, Hanna E. author.
Other author	Ables, Elizabeth Tweedie, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2017.
Description	93 pages : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	Hormone receptors. Drosophila melanogaster. Stem cells. Ecdysone.

Summary	Stem cells are a fundamental underpinning of tissue biology. Loss of the self-renewing function of stem cells leads to conditions such as infertility and tissue wasting. Stem cells integrate a variety of signals to maintain their fate and proliferative capacity. Although intrinsic and local cues are well studied, less is known about how extrinsic signals, such as hormones, affect stem cell fate and function. The highly characterized Drosophila melanogaster steroid hormone ecdysone regulates germline stem cell (GSC) proliferation and self-renewal, as well as oogenesis and metamorphosis. Though many genes, including nuclear hormone receptor ftz transcription factor 1 (ftz-f1), are thought to be targets of ecdysone signaling, it is unclear how these targets impact GSC fate and function. To explore the role of ftz-f1 in ovarian stem cells, we used the UAS-GAL4 system and RNA interference (RNAi) to reduce ftz-f1 function specifically in germ cells or surrounding somatic cells. We demonstrate that ftz-f1 is intrinsically required for the establishment of the proper number of GSCs during development. Reduced ftz-f1 function in germ cells leads to a significant decrease in average number of GSCs. During larval stages, ftz-f1 depleted ovaries contain a number of PGCs located significantly further away from the terminal filament stacks. Our results also suggest that ftz-f1 is required in ovarian somatic cells during development for proper movement of germ cells out of the germarium in adult stages. Taken together, we suggest ftz-f1 function during juvenile stages is critical for the establishment of GSCs and development of their progeny.
General note	Presented to the faculty of the Department of Biology
General note	Advisor: Elizabeth Ables
General note	Title from PDF t.p. (viewed January 2, 2018).
Dissertation note	M.S. East Carolina University 2017
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Drosophila ovarian stem cell establishment is regulated by nuclear hormone receptor ftz-f1 / by Hanna E. Berghout.

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