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001 ocn857962980;

003 OCoLC;

005 20160429093825.0;

008 130905t20142014nyua b 001 0 eng ;

020 a| 9781627037020 (alk. paper);

020 a| 1627037020 (alk. paper);

020 z| 9781627037037 (eBook);

035 a| (Sirsi) 99965524626;

035 a| 99965524626;

035 a| (OCoLC)857962980;

042 a| nlmcopyc;

050 4 a| QP623b| .M57 2014;

060 00 a| W1b| ME9616J v.1095 2014;

060 10 a| QU 25;

082 04 a| 572.9b| 23;

245 00 a| miRNA maturation :b| methods and protocols /c| edited by Christoph Arenz.;

246 1 a| Micro RNA maturation;

264 1 a| New York :b| Humana Press :b| Springer,c| [2014];

300 a| x, 192 pages :b| illustrations (some colored) ;c| 26 cm.;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

490 1 a| Methods in molecular biology,x| 1064-3745 ;v| 1095;

490 1 a| Springer protocols;

504 a| Includes bibliographical references and index.;

505 00 t| Pathway of miRNA maturation /r| Michael Sand --t| MicroRNA maturation and human disease /r| Marlen Hesse and Christoph Arenz --t| Approaches to the modulation of miRNA maturation /r| Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young --t| Expression profiling of components of the miRNA maturation machinery /r| Michael Sand and Marina Skrygan --t| Primary MicroRNA processing assay reconstituted using recombinant Drosha and DGCR8 /r| Ian Barr and Feng Guo --t| In vivo processing assay based on a dual-luciferase reporter system to evaluate DROSHA enzymatic activity /r| Vera Bilan ... [et al.] --t| Assaying dicer-mediated miRNA maturation by means of fluorescent Substrates /r| Marlen Hesse, Brian P. Davies, and Christoph Arenz --t| Fluorescence correlation spectroscopy-based enzyme assay for human dicer /r| Eileen Magbanua and Ulrich Hahn --t| Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification /r| Saskia Neubacher and Christoph Arenz --t| Quantitative RT-PCR specific for precursor and mature miRNAs /r| Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj --t| Cellular microRNA sensors based on luciferase reporters /r| Colleen M. Connelly and Alexander Deiters --t| Identification of inhibitors of microRNA function from small molecule screens /r| Colleen M. Connelly and Alexander Deiters --t| Inhibition of miRNA maturation by peptide nucleic acids /r| Concetta Avitabile ... [et al.] --t| Molecular methods for validation of the biological activity of peptide nucleic acids targeting microRNAs /r| Eleonora Brognara ... [et al.] --t| Lentiviral overexpression of miRNAs /r| Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj.;

520 a| In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.;

650 0 a| RNAv| Laboratory manuals.=| ^A4131;

650 7 a| RNA.2| fast0| (OCoLC)fst01086244;

655 7 a| Laboratory Manuals.2| mesh;

655 7 a| Handbooks, manuals, etc.2| fast0| (OCoLC)fst01423877;

700 1 a| Arenz, Christoph,e| editor.=| ^A1284916;

830 0 a| Methods in molecular biology (Clifton, N.J.)v| v. 1095.x| 1064-3745=| ^A682607;

830 0 a| Springer protocols (Series)x| 1949-2448=| ^A997552;

949 i| 30372016604586o| jjlm;

960 o| 1s| 119.00t| Joyner48u| JBIOz| USD;

596 a| 1;

998 a| 4350215;

999 a| QP623 .M57 2014w| LCc| 1i| 30372016604586d| 4/12/2016e| 2/17/2016l| JGESm| JOYNERr| Ys| Yt| JGESBKu| 2/16/2016x| BOOKz| JSTACKSo| .STAFF. jjlm;

miRNA maturation : methods and protocols / edited by Christoph Arenz.

Other author	Arenz, Christoph, editor.
Format	Archival & Manuscript Material
Publication	New York : Humana Press : Springer, [2014]
Copyright Date	©2014
Description	x, 192 pages : illustrations (some colored) ; 26 cm.
Subjects	RNA--Laboratory manuals.

Variant title	Micro RNA maturation
Series	Methods in molecular biology, 1064-3745 ; 1095 Springer protocols Methods in molecular biology (Clifton, N.J.) v. 1095. 1064-3745 ^A682607 Springer protocols (Series) 1949-2448 ^A997552
Contents	Pathway of miRNA maturation / Michael Sand -- MicroRNA maturation and human disease / Marlen Hesse and Christoph Arenz -- Approaches to the modulation of miRNA maturation / Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young -- Expression profiling of components of the miRNA maturation machinery / Michael Sand and Marina Skrygan -- Primary MicroRNA processing assay reconstituted using recombinant Drosha and DGCR8 / Ian Barr and Feng Guo -- In vivo processing assay based on a dual-luciferase reporter system to evaluate DROSHA enzymatic activity / Vera Bilan ... [et al.] -- Assaying dicer-mediated miRNA maturation by means of fluorescent Substrates / Marlen Hesse, Brian P. Davies, and Christoph Arenz -- Fluorescence correlation spectroscopy-based enzyme assay for human dicer / Eileen Magbanua and Ulrich Hahn -- Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification / Saskia Neubacher and Christoph Arenz -- Quantitative RT-PCR specific for precursor and mature miRNAs / Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj -- Cellular microRNA sensors based on luciferase reporters / Colleen M. Connelly and Alexander Deiters -- Identification of inhibitors of microRNA function from small molecule screens / Colleen M. Connelly and Alexander Deiters -- Inhibition of miRNA maturation by peptide nucleic acids / Concetta Avitabile ... [et al.] -- Molecular methods for validation of the biological activity of peptide nucleic acids targeting microRNAs / Eleonora Brognara ... [et al.] -- Lentiviral overexpression of miRNAs / Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj.
Abstract	In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.
Bibliography note	Includes bibliographical references and index.
Genre/form	Laboratory Manuals.
Genre/form	Handbooks, manuals, etc.
ISBN	9781627037020 (alk. paper)
ISBN	1627037020 (alk. paper)

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miRNA maturation : methods and protocols / edited by Christoph Arenz.

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