Reconstituting the cytoskeleton / edited by Ronald D. Vale.
| Other author | Vale, Ronald. |
| Format | Electronic |
| Edition | First edition. |
| Publication Info | Amsterdam, Netherlands ; Boston, Mass. : Elsevier/Academic Press, 2014. |
| Description | xxiii, 484 pages, 30 unnumbered pages of plates : illustrations (some color) ; 24 cm. |
| Supplemental Content | Full text available from Methods in Enzymology |
| Supplemental Content | Full text available from ScienceDirect Enzymology Book Series |
| Subjects |
| Series | Methods in enzymology, 0076-6879 ; volume 540 Methods in enzymology v. 540. 0076-6879 ^A497553 |
| Contents | Actin filament dynamics using microfluidics -- Bacterial actin-like proteins: purification and characterization of self-assembly properties -- Quantitative analysis of microtubule self-assembly kinetics and tip structure -- Biochemical reconstitution of the WAVE regulatory complex -- Rotational movement of formins evaluated by using single-molecule fluorescence polarization -- Single-molecule studies of actin assembly and disassembly factors -- Assaying microtubule nucleation of the [gamma]-Tubulin ring complex -- Reconstituting dynamic microtubule polymerization regulation by TOG domain proteins -- Generation of differentially modified microtubules using In Vitro enzymatic approaches -- Engineering defined motor ensembles with DNA origami -- Construction and analyses of elastically coupled multiple-motor systems -- Reconstitution of cortical dynein function -- Reconstitution of microtubule-dependent organnelle transport -- Reconstituting the motility of isolated intracellular cargoes -- Reconstitution of contractile actomyosin arrays -- Directed actin assembly and motility -- In Vitro reconstitution of dynamic microtubules interacting with actin filament networks -- Measuring kinetochore-microtubule interaction In Vitro -- Micropattern-guided assembly of overlapping pairs of dynamic microtubules -- WAVE regulatory complex activation -- Dissecting principles governing actin assembly using yeast extracts -- Xenopus egg ctyoplasm with intact actin -- Glycogen-supplemented mitotic cytosol for analyzing xenopus egg microtubule organization -- Spindle assembly on immmobilized chromatin micropatterns. |
| Bibliography note | Includes bibliographical references and indexes. |
| Access restriction | Available only to authorized users. |
| Technical details | Mode of access: World Wide Web |
| Genre/form | Electronic books. |
| ISBN | 9780123979247 |
| ISBN | 0123979242 |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Electronic Resources | ✔ Available |