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LEADER 03869ctm a2200613 i 4500

001 ocm25763937;

003 OCoLC;

005 20260722082022.0;

008 920505s1991 xx af bm 000 0 eng d;

035 a| (Sirsi) o25763937;

035 a| (OCoLC)25763937;

049 a| EREE;

050 4 a| QM611b| .S55x 1991;

100 1 a| Simonds, Lawrence J.,e| author.?| UNAUTHORIZED;

245 10 a| Evidence of prolactin transport across human fetal membranes /c| by Lawrence J. Simonds.;

264 0 c| 1991.;

300 a| 35 leaves, 9 unnumbered leaves of plates :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1991;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Charles A. Singhas;

520 3 a| During human gestation, transformed endometrial stromal cells referred to as the decidua synthesize and release large quantities of prolactin which is transported unidirectionally across the amniochorionic membranes and into the amniotic fluid surrounding the fetus. Although the decidual origin of amniotic fluid prolactin is currently accepted based upon biochemical evidence, immunohistochemical techniques used to localize prolactin within the fetal membranes have produced conflicting results, as have attempts to examine the nature of prolactin transport. The present study utilizes immunohistochemistry and electron microscopic techniques to examine the distribution of prolactin within the amnio chorionic membrane complex. Prolactin was localized within the decidua, chorion, and amniotic epithelium indicating transport across the fetal membranes. Ultrastructural studies suggest that decidual cells may release prolactin by exocytosis into the extracellular matrix for transport. Intense nuclear staining with antibodies specific for human prolactin was also revealed in cells located in the deep decidua. The significance of this staining remains unclear.;

504 a| Includes bibliographical references (leaves 33-35).;

650 0 a| Amniotic liquidx| Examination.=| ^A286223;

650 0 a| Prolactin.=| ^A6685;

650 0 a| Decidua.=| ^A286224;

650 0 a| Fetusx| Research.=| ^A2428;

650 0 a| Immunohistochemistry.=| ^A201521;

650 2 a| Prolactin.0| (DNLM)D011388=| ^A948796;

650 2 a| Decidua.0| (DNLM)D003656=| ^A1492009;

650 2 a| Immunohistochemistry.0| (DNLM)D007150=| ^A952031;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Amniotic liquidx| Examination.2| fast0| (OCoLC)fst00807831;

650 7 a| Decidua.2| fast0| (OCoLC)fst00888991;

650 7 a| Fetusx| Research.2| fast0| (OCoLC)fst00923470;

650 7 a| Immunohistochemistry.2| fast0| (OCoLC)fst00967974;

650 7 a| Prolactin.2| fast0| (OCoLC)fst01078859;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Singhas, Charles A.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12763;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 417191;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 665849d| 9/5/2001l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 9/5/2001x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 417191-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 5/28/2026x| ETDz| JERESOURCE;

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Evidence of prolactin transport across human fetal membranes / by Lawrence J. Simonds.

Author/creator	Simonds, Lawrence J. author.
Other author	Singhas, Charles A., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1991.
Description	35 leaves, 9 unnumbered leaves of plates : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Amniotic liquid--Examination. Prolactin. Decidua. Fetus--Research. Immunohistochemistry. Prolactin. Decidua. Immunohistochemistry.

Summary	During human gestation, transformed endometrial stromal cells referred to as the decidua synthesize and release large quantities of prolactin which is transported unidirectionally across the amniochorionic membranes and into the amniotic fluid surrounding the fetus. Although the decidual origin of amniotic fluid prolactin is currently accepted based upon biochemical evidence, immunohistochemical techniques used to localize prolactin within the fetal membranes have produced conflicting results, as have attempts to examine the nature of prolactin transport. The present study utilizes immunohistochemistry and electron microscopic techniques to examine the distribution of prolactin within the amnio chorionic membrane complex. Prolactin was localized within the decidua, chorion, and amniotic epithelium indicating transport across the fetal membranes. Ultrastructural studies suggest that decidual cells may release prolactin by exocytosis into the extracellular matrix for transport. Intense nuclear staining with antibodies specific for human prolactin was also revealed in cells located in the deep decidua. The significance of this staining remains unclear.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Charles A. Singhas
Dissertation note	M.S. East Carolina University 1991
Bibliography note	Includes bibliographical references (leaves 33-35).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Evidence of prolactin transport across human fetal membranes / by Lawrence J. Simonds.

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