Phage-associated lysozyme of Staphylococcus Aureus : its characterization and use in genetic transformation / by Joan M. Nippert.
| Author/creator | Nippert, Joan Mae author. |
| Other author | Allen, Wendall E., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1974. |
| Description | 56 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Attempts were made to use phage-associated lysozyme (PAL) obtained from cells of Staphylococcus aureus strain PS53 (International Phage-Typing Series) lysed by staphylococcal phage 53 to obtain biologically active DNA to be used in the transformation of tetracycline resistance and DNase activity. Characterization of PAL included determination of: the effect of reducing agents on activity, the optimal conditions of protein precipitation, the effect of age and storage conditions on activity, and its lytic activity on various strains of aureus alone and in combination with other physical and chemical treatments. Using 1-methyl-3-nitro-1-nitrosoguanidine, mutants having either increased or decreased nuclease activity were isolated. A mutant resistant to tetracycline and having increased nuclease activity was selected as a donor strain and a mutant sensitive to tetracycline and having decreased nuclease activity was selected as a recipient strain. The transformation of neither of these characteristics was observed in any of the experiments attempted. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Wendall E. Allen |
| Dissertation note | M.A. East Carolina University 1974 |
| Bibliography note | Includes bibliographical references (leaves 53-56). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |