Reconstruction of single comb from dissociated cells on the chorioallantoic membrane / by Rosalie Marie Vogel.
| Author/creator | Vogel, Rosalie M., 1943- author. |
| Other author | Lawrence, Irvin E., Jr., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1967. |
| Description | 27 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Reaggregation of White Leghorn single comb cells was studied in culture. Entire comb organs from 8 and 10 day incubated chicks were excised and dissociated in a calcium-magnesium-free trypsin solution. The dissociated cells were washed in Tyrode's solution, centrifuged, transferred by pipette to the chorioallantois of host chicks, and developed for approximately 10 days. Some comb structure was reconstructed in the grafts. Two organ patterns formed; elongate ridge and irregular points. Several tissues developed. Typical comb epidermis formed in many grafts. Dense regular dermis and a comb-like vascular pattern formed in all grafts. Loose irregular tissue was frequent. Several grafts formed non-comb tissues of cartilage and bone. Tissue interactions between epidermis and dermis and between cartilage and dermis were apparent. Where thin epidermis formed on the free margin of the comb ridge, underlying dermis was usually organized in typical comb pattern. Cartilage formed an elongate rod, and when present, comb ridge was prominent and the vascular and dermal patterns were most typical. There was evidence that mass of tissue was significant: cartilage formed in all whole comb cellular suspensions, but was missing in most of the partial combs. Comb mesenchymal cells possess some pleuripotency; a capacity expressed in formation of cartilage and bone. Pleuripotency did not include feather papillae. Since comb is an integumentary derivative, the lack of feather formation in any of the grafts is considered significant. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Irvin E. Lawrence, Jr. |
| Dissertation note | M.A. East Carolina College 1967 |
| Bibliography note | Includes bibliographical references (leaves 22-23). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |