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020 a| 9780123737496 (alk. paper);

020 a| 0123737494 (alk. paper);

035 a| (WaSeSS)ssj0000097613;

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245 00 a| Advanced bacterial geneticsh| [electronic resource] :b| use of transposons and phage for genomic engineering /c| edited by Kelly T. Hughes, Stanley R. Maloy.;

260 a| Amsterdam ;a| Boston :b| Elsevier / Academic Press,;

300 a| xxxiv, 284 p. :b| ill.;

490 1 a| Methods in enzymology ;v| v. 421;

504 a| Includes bibliographical references and indexes.;

505 0 a| Strain collections and genetic nomenclature / Stanley R. Maloy and Kelly T. Hughes -- Use of antibiotic-resistant transposons for mutagenesis / Stanley R. Maloy -- In vivo mutagenesis using EZ-Tn5 / John R. Kirby -- Identification of essential genes in bacteria / David R. Hillyard and Michael J. Reed -- Isolation of transposon insertions / Stanley R. Maloy -- Localized mutagenesis / Stanley R. Maloy -- Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / Kelly T. Hughes -- Target-directed proteolysis in vivo / Markus Eser ... [et al.] -- Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / Beth Traxler and Eliora Gachelet -- Using genomic microarrays to study insertional/transposon mutant libraries / David N. Baldwin and Nina R. Salama -- Screening transposon mutant libraries using full-genome oligonucleotide microarrays / Kelly M. Winterberg and William S. Reznikoff -- Creating recombination-activated genes and sequence-defined mutant libraries using transposons / Larry Gallagher ... [et al.] -- Use of operon and gene fusions to study gene regulation in Salmonella / Kelly T. Hughes and Stanley R. Maloy -- Genomic screening for regulatory genes using the T-POP transposon / Changhan Lee ... [et al.] -- Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / James A. Sawitzke ... [et al.] -- [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / Joyce E. Karlinsey -- Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / Nathalie Garcia-Russell, Samantha S. Orchard, and Anca M. Segall -- Dissecting nucleic acid-protein interactions using challenge phage / Stanley R. Maloy and Jeffrey Gardner -- Mud-P22 / Jenny A. Crain and Stanley R. Maloy -- Phage metagenomics / Veronica Casas and Forest Rowher.;

506 a| Available only to authorized users.;

530 a| Also issued online.;

538 a| Mode of access: World Wide Web;

650 12 a| Bacteriax| genetics=| ^A922824;

650 22 a| DNA, Bacterial=| ^A1202461;

655 0 a| Electronic books.=| ^A491897;

700 1 a| Hughes, Kelly Thomas.?| UNAUTHORIZED;

700 1 a| Maloy, Stanley R.=| ^A954417;

830 0 a| Methods in enzymologyv| v. 421.=| ^A497553;

856 40 z| Full text available from Methods in Enzymologyu| https://go.openathens.net/redirector/ecu.edu?url=http%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Fbook%2F9780123737496;

856 40 z| Full text available from ScienceDirect Enzymology Book Seriesu| https://go.openathens.net/redirector/ecu.edu?url=https%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Fbook%2F9780123737496;

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Advanced bacterial genetics use of transposons and phage for genomic engineering / edited by Kelly T. Hughes, Stanley R. Maloy.

Other author	Hughes, Kelly Thomas.
Other author	Maloy, Stanley R.
Format	Electronic
Publication Info	Amsterdam ; Boston : Elsevier / Academic Press,
Description	xxxiv, 284 p. : ill.
Supplemental Content	Full text available from Methods in Enzymology
Supplemental Content	Full text available from ScienceDirect Enzymology Book Series
Subjects	Bacteria--genetics. DNA, Bacterial

Series	Methods in enzymology ; v. 421 Methods in enzymology v. 421. ^A497553
Contents	Strain collections and genetic nomenclature / Stanley R. Maloy and Kelly T. Hughes -- Use of antibiotic-resistant transposons for mutagenesis / Stanley R. Maloy -- In vivo mutagenesis using EZ-Tn5 / John R. Kirby -- Identification of essential genes in bacteria / David R. Hillyard and Michael J. Reed -- Isolation of transposon insertions / Stanley R. Maloy -- Localized mutagenesis / Stanley R. Maloy -- Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / Kelly T. Hughes -- Target-directed proteolysis in vivo / Markus Eser ... [et al.] -- Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / Beth Traxler and Eliora Gachelet -- Using genomic microarrays to study insertional/transposon mutant libraries / David N. Baldwin and Nina R. Salama -- Screening transposon mutant libraries using full-genome oligonucleotide microarrays / Kelly M. Winterberg and William S. Reznikoff -- Creating recombination-activated genes and sequence-defined mutant libraries using transposons / Larry Gallagher ... [et al.] -- Use of operon and gene fusions to study gene regulation in Salmonella / Kelly T. Hughes and Stanley R. Maloy -- Genomic screening for regulatory genes using the T-POP transposon / Changhan Lee ... [et al.] -- Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / James A. Sawitzke ... [et al.] -- [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / Joyce E. Karlinsey -- Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / Nathalie Garcia-Russell, Samantha S. Orchard, and Anca M. Segall -- Dissecting nucleic acid-protein interactions using challenge phage / Stanley R. Maloy and Jeffrey Gardner -- Mud-P22 / Jenny A. Crain and Stanley R. Maloy -- Phage metagenomics / Veronica Casas and Forest Rowher.
Bibliography note	Includes bibliographical references and indexes.
Access restriction	Available only to authorized users.
Other forms	Also issued online.
Technical details	Mode of access: World Wide Web
Genre/form	Electronic books.
ISBN	9780123737496 (alk. paper)
ISBN	0123737494 (alk. paper)

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Library	Location	Call Number	Status	Item Actions
	Electronic Resources	Online Content Full text available from Methods in Enzymology Full text available from ScienceDirect Enzymology Book Series	✔ Available

Advanced bacterial genetics use of transposons and phage for genomic engineering / edited by Kelly T. Hughes, Stanley R. Maloy.

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