Localization of the bacteriophage P1 cin recombinase promoter / by David L. Onks.
| Author/creator | Onks, David L. author. |
| Other author | Kennedy, Kathleen E., 1946- degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1990. |
| Description | [iv], 62 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Site-specific inversion is a mechanism allowing an organism to express alternate phenotypes. Inversion frequency of a DNA segment may be under the control of the recombinase promoter. Promoter mapping studies, including endonuclease mapping and primer extension, localize the promoter of the bacteriophage P1 cin recombinase to a region less than 80 base pairs from the start codon. The endonuclease mapping gels were scanned using a densitometer to quantitate inversion relative to the parent plasmid. It was shown that additional promoters outside the invertible segment affect the frequency of inversion. One orientation of the invertible segment is favored as opposed to the other. Site-specific inversion systems could ultimately be used as promoter-probe vectors. These vectors would identify weak promoters cloned upstream from the recombinase gene. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Kathleen E. Kennedy |
| Dissertation note | M.S. East Carolina University 1990 |
| Bibliography note | Includes bibliographical references (leaves 60-62). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Joyner | Microforms | MICROFILM | ✔ Available | |
| Electronic Resources | Access Content Online | ✔ Available |