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003 OCoLC;

005 20151028113904.0;

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007 cr bn|||||||||;

008 150901s2015 ncua ob 000 0 eng d;

035 a| (Sirsi) o919514855;

035 a| (OCoLC)919514855;

049 a| EREE;

100 1 a| Mamillapalli, Srivalli Swathi,e| author.?| UNAUTHORIZED;

245 10 a| Genetic control of cell fate specification in Caenorhabditis elegans germline /c| by Srivalli Swathi Mamillapalli.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2015.;

300 a| 75 pages :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 1018.0Kb2| rda;

490 1 a| ECU Brody School of Medicine thesis;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2015.;

500 a| Presented to the faculty of the Department Biomedical Sciences.;

500 a| Advisor: Myon-Hee Lee.;

500 a| Title from PDF t.p. (viewed August 28, 2015).;

520 3 a| The precise regulation of germ cell fates (sperm or oocyte) lies at the heart of reproduction and fertility. The nematode Caenorhabditis elegans hermaphrodites produce a discrete number of sperm during larval development and then switch to produce oocyte during adulthood. A number of positive (e.g., fbf genes) and negative (e.g., gld-3) regulators are important for this switch. Here, we found that aberrant activation of MPK-1 (an ERK homolog) by removal of both fbf-1 and lip-1 partially inhibits sperm-oocyte switch, resulting in Mog (masculinization of germline) sterility. The fbf-1 gene encodes a conserved PUF (Pumilio and FBF) RNA-binding protein and the lip-1 gene encodes an MPK-1/ERK phosphatase. Notably, inhibition of MPK-1/ERK signaling by either genetic mutation or chemical inhibition reprograms the germ cell fate and thus helps in regaining the fertility. We also found that fbf-1; lip-1 Mog sterility was enhanced by the depletion of G2/M cell cycle regulators, including CYB-3/Cyclin B, CDK-1/CDK1, and CDC-25.1/CDC25. Markedly, cdc-25.1 mRNA is a direct target of FBF-1. These results suggest that FBF-1 and LIP-1 may promote sperm-oocyte switch by activating MPK-1/ERK signaling and G2/M cell cycle progression.;

504 a| Includes bibliographical references.;

650 2 a| Spermatogenesisx| physiology.=| ^A931767;

650 2 a| Disorders of Sex Developmentx| metabolism.=| ^A1273093;

650 2 a| Disorders of Sex Developmentx| genetics.=| ^A1273092;

650 2 a| Caenorhabditis elegansx| growth & development.=| ^A1273107;

650 2 a| Caenorhabditis elegansx| metabolism.=| ^A1195256;

650 2 a| RNA-Binding Proteinsx| metabolism.=| ^A1195255;

653 a| Developmental Biology;

653 a| Genetics Medicine;

653 a| C.elegans;

653 a| Cell Cycle Regulation;

653 a| Cell Fate Specification;

653 a| Genetic Control;

653 a| Germline;

653 a| MPK-1/ERK Signaling;

700 1 a| Lee, Myon-Hee,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| Brody School of Medicine.b| Office of Research and Graduate Studies.b| Biomedical Sciences.?| UNAUTHORIZED;

830 0 a| ECU Brody School of Medicine thesis.=| ^A964744;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/5019;

949 o| jdjr;

994 a| C0b| ERE;

096 a| WQ 205;

596 a| 1 4;

998 a| 3808267;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3808267-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/1/2015x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3808267-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/1/2015x| ETDz| HERESOURCE;

Genetic control of cell fate specification in Caenorhabditis elegans germline / by Srivalli Swathi Mamillapalli.

Author/creator	Mamillapalli, Srivalli Swathi author.
Other author	Lee, Myon-Hee, degree supervisor.
Other author	Brody School of Medicine. Office of Research and Graduate Studies. Biomedical Sciences.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2015.
Description	75 pages : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Spermatogenesis--physiology. Disorders of Sex Development--metabolism. Disorders of Sex Development--genetics. Caenorhabditis elegans--growth & development. Caenorhabditis elegans--metabolism. RNA-Binding Proteins--metabolism.

Series	ECU Brody School of Medicine thesis ECU Brody School of Medicine thesis. ^A964744
Summary	The precise regulation of germ cell fates (sperm or oocyte) lies at the heart of reproduction and fertility. The nematode Caenorhabditis elegans hermaphrodites produce a discrete number of sperm during larval development and then switch to produce oocyte during adulthood. A number of positive (e.g., fbf genes) and negative (e.g., gld-3) regulators are important for this switch. Here, we found that aberrant activation of MPK-1 (an ERK homolog) by removal of both fbf-1 and lip-1 partially inhibits sperm-oocyte switch, resulting in Mog (masculinization of germline) sterility. The fbf-1 gene encodes a conserved PUF (Pumilio and FBF) RNA-binding protein and the lip-1 gene encodes an MPK-1/ERK phosphatase. Notably, inhibition of MPK-1/ERK signaling by either genetic mutation or chemical inhibition reprograms the germ cell fate and thus helps in regaining the fertility. We also found that fbf-1; lip-1 Mog sterility was enhanced by the depletion of G2/M cell cycle regulators, including CYB-3/Cyclin B, CDK-1/CDK1, and CDC-25.1/CDC25. Markedly, cdc-25.1 mRNA is a direct target of FBF-1. These results suggest that FBF-1 and LIP-1 may promote sperm-oocyte switch by activating MPK-1/ERK signaling and G2/M cell cycle progression.
General note	Presented to the faculty of the Department Biomedical Sciences.
General note	Advisor: Myon-Hee Lee.
General note	Title from PDF t.p. (viewed August 28, 2015).
Dissertation note	M.S. East Carolina University 2015.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Genetic control of cell fate specification in Caenorhabditis elegans germline / by Srivalli Swathi Mamillapalli.

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