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001 ocn869832902;

003 OCoLC;

005 20140310011119.0;

006 m o d ;

007 cr bn|||||||||;

008 140204s2013 ncua ob 000 0 eng d;

035 a| (Sirsi) o869832902;

035 a| (OCoLC)869832902;

049 a| EREE;

090 a| TP681;

100 1 a| Winfrey, William Brandon,e| author.?| UNAUTHORIZED;

245 10 a| Inhibitory and oxidative effects of gossypol on MCF7 breast cancer cells in vitro /c| by William Brandon Winfrey.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2013.;

300 a| 108 pages :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 2.63Mb2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2013.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Baohong Zhang.;

500 a| Title from PDF t.p. (viewed February 25, 2014).;

520 3 a| Human cancer is the second leading cause of death in the United States and breast cancer is responsible for the second highest number of deaths in women with cancer worldwide. Today, cancer is becoming more and more resistant to current chemotherapeutic agents. In an effort to decrease this resistance, natural products like gossypol are being tested for efficacy as a natural chemotherapeutic agent with anti-cancer properties. Current literature demonstrates that gossypol is indeed an effective drug against breast cancer when used alone and when combined with other chemotherapeutic agents [1-5]. The majority of current literature focuses on the ability of gossypol to antagonize anti-apoptotic proteins like BCL-XL and induce apoptosis [6, 7]. This study helps understand previous data and goes beyond the current knowledge base and explores not only apoptosis induction, but also on other important effects like: oxidative stress, other possible avenues of cell death, growth and development, and cell cycle progression. Combining physiological, genotypic, flow cytometric and biochemical assays, a more complete understanding of gossypol's efficacy and mechanism of action can be ascertained. In past studies, the focus of gossypol's efficacy has been too narrow and currently the study of gene regulators like microRNAs (miRNAs) has not been incorporated. This study reveals evidence that miRNA may play an important role and that gossypol's efficacy is in fact a multi-component system that is interconnected in its overall mechanism of action.;

504 a| Includes bibliographical references.;

650 0 a| Gossypol.=| ^A988501;

650 0 a| Breastx| Cancer.=| ^A2411;

650 0 a| Chemotherapy.=| ^A1403;

653 a| Biology;

700 1 a| Zhang, Baohonge| degree supervisor.=| ^A1031945;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/4325;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3422642;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3422642-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 2/4/2014x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3422642-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 2/4/2014x| ETDz| HERESOURCE;

Inhibitory and oxidative effects of gossypol on MCF7 breast cancer cells in vitro / by William Brandon Winfrey.

Author/creator	Winfrey, William Brandon author.
Other author	Zhang, Baohong degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2013.
Description	108 pages : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Gossypol. Breast--Cancer. Chemotherapy.

Summary	Human cancer is the second leading cause of death in the United States and breast cancer is responsible for the second highest number of deaths in women with cancer worldwide. Today, cancer is becoming more and more resistant to current chemotherapeutic agents. In an effort to decrease this resistance, natural products like gossypol are being tested for efficacy as a natural chemotherapeutic agent with anti-cancer properties. Current literature demonstrates that gossypol is indeed an effective drug against breast cancer when used alone and when combined with other chemotherapeutic agents [1-5]. The majority of current literature focuses on the ability of gossypol to antagonize anti-apoptotic proteins like BCL-XL and induce apoptosis [6, 7]. This study helps understand previous data and goes beyond the current knowledge base and explores not only apoptosis induction, but also on other important effects like: oxidative stress, other possible avenues of cell death, growth and development, and cell cycle progression. Combining physiological, genotypic, flow cytometric and biochemical assays, a more complete understanding of gossypol's efficacy and mechanism of action can be ascertained. In past studies, the focus of gossypol's efficacy has been too narrow and currently the study of gene regulators like microRNAs (miRNAs) has not been incorporated. This study reveals evidence that miRNA may play an important role and that gossypol's efficacy is in fact a multi-component system that is interconnected in its overall mechanism of action.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Baohong Zhang.
General note	Title from PDF t.p. (viewed February 25, 2014).
Dissertation note	M.S. East Carolina University 2013.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Inhibitory and oxidative effects of gossypol on MCF7 breast cancer cells in vitro / by William Brandon Winfrey.

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