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001 ocn857590018;

003 OCoLC;

005 20131120121716.0;

006 m o d ;

007 cr bn|||||||||;

008 130904s2013 ncua ob 000 0 eng d;

035 a| (Sirsi) o857590018;

035 a| (OCoLC)857590018;

049 a| EREE;

090 a| QP572.G3;

100 1 a| White, Daniele| author.=| ^A480991;

245 14 a| The effects of gastrin and Hox-A1 on HT-29 cell proliferation /c| by Daniel White.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2013.;

300 a| 57 pages :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 3.95Mb2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2013.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Jean-Luc Scemama.;

500 a| Title from PDF t.p. (viewed October 1, 2013).;

520 3 a| Gastrin is a gastrointestinal peptide hormone synthesized in G cells and secreted in the antrum of the stomach. Though its primary role is to stimulate the secretion of gastric acid by parietal cells, it is also involved in the maintenance of cellular identity and proliferation of gastrointestinal tissue in the stomach, pancreas, and duodenum. Aberrant expression of gastrin has been associated with increased rates of cancer, and more specifically colon cancer. While some of the effects are due to the mature amidated form of the peptide, G17-NH2, its incompletely processed precursors have been shown to have oncogenic functions as well. In this investigation, we focus on glycine-extended gastrin 17 (G17-Gly), one of the most characterized precursors, and how its role in cellular proliferation compares to G17-NH2. Both forms can induce cell proliferation; however, the mechanisms by which these peptides act are not well defined. In this study, we explored the relationship of both gastrin forms with respect to Hox-A1, a homeodomain containing transcription factor known to be important in cell growth and differentiation. G17-NH2 (+44%) and G17-Gly (+35%) significantly increased cell proliferation in the human colon adenocarcinoma cell line, HT-29. Quantitative real time polymerase chain reaction (qRT-PCR) and western blot analysis indicated that these effects were not associated with altered Hox-A1 expression. Lastly, RNA interference suggested that HT-29 and gastrin-stimulated growth were not dependent on Hox-A1.;

504 a| Includes bibliographical references.;

650 0 a| Gastrin.=| ^A842512;

650 0 a| Colon (Anatomy)x| Cancer.=| ^A230293;

653 a| Biology;

700 1 a| Scemama, Jean-Luc,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/4187;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3362004;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3362004-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/4/2013x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3362004-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/4/2013x| ETDz| HERESOURCE;

The effects of gastrin and Hox-A1 on HT-29 cell proliferation / by Daniel White.

Author/creator	White, Daniel author.
Other author	Scemama, Jean-Luc, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2013.
Description	57 pages : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Gastrin. Colon (Anatomy)--Cancer.

Summary	Gastrin is a gastrointestinal peptide hormone synthesized in G cells and secreted in the antrum of the stomach. Though its primary role is to stimulate the secretion of gastric acid by parietal cells, it is also involved in the maintenance of cellular identity and proliferation of gastrointestinal tissue in the stomach, pancreas, and duodenum. Aberrant expression of gastrin has been associated with increased rates of cancer, and more specifically colon cancer. While some of the effects are due to the mature amidated form of the peptide, G17-NH2, its incompletely processed precursors have been shown to have oncogenic functions as well. In this investigation, we focus on glycine-extended gastrin 17 (G17-Gly), one of the most characterized precursors, and how its role in cellular proliferation compares to G17-NH2. Both forms can induce cell proliferation; however, the mechanisms by which these peptides act are not well defined. In this study, we explored the relationship of both gastrin forms with respect to Hox-A1, a homeodomain containing transcription factor known to be important in cell growth and differentiation. G17-NH2 (+44%) and G17-Gly (+35%) significantly increased cell proliferation in the human colon adenocarcinoma cell line, HT-29. Quantitative real time polymerase chain reaction (qRT-PCR) and western blot analysis indicated that these effects were not associated with altered Hox-A1 expression. Lastly, RNA interference suggested that HT-29 and gastrin-stimulated growth were not dependent on Hox-A1.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Jean-Luc Scemama.
General note	Title from PDF t.p. (viewed October 1, 2013).
Dissertation note	M.S. East Carolina University 2013.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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