Polyclonal and monoclonal antibodies that define an IgA associated idiotype / by William Bruce Tannehill.
| Author/creator | Tannehill, William Bruce author. |
| Other author | Smith, A. Mason, degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1987. |
| Description | v, 88 leaves : 13 illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Subject | Mineral Oil Plasmacytoma (MOPC) 467 is a monoclonal IgA producing plasmacytoma which was induced in BALB/c mice. Purified MOPC 467 IgA and IgA light chain were used to immunize New Zealand white rabbits and A/HeJ mice in order to produce anti-MOPC 467 idiotypic antisera. Anti-MOPC 467 rabbit antisera were purified using affinity chromatography. The polyclonal antisera were tested for MOPC 467 idiotype specificity using a competitive enzyme linked immunosorbent assay (ELISA). Hybridoma techniques were used to fuse spleen cells from immunized mice with FOX-NY myeloma cells. Hybridomas producing anti-MOPC 467 antibody were cloned for the production of monoclonal anti-MOPC 467. The monoclonal antisera were purified using affinity chromatography and clonotyped using enzyme linked immunosorbent assay (ELISA). Anti-MOPC 467 idiotype specificity of the monoclonal antibodies was tested using a competitive ELISA. The anti-MOPC 467 polyclonal and monoclonal antibodies produced in this study defined, through their specificity, the location of the MOPC 467 idiotype as being on the IgA light chain and within the antibody binding site, or para- tope. A discussion of the importance of anti-idiotypic antisera in medical research and possible future studies using anti-MOPC 467 idiotypic antisera is presented. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: A. Mason Smith |
| Dissertation note | M.S. East Carolina University 1987 |
| Bibliography note | Includes bibliographical references (leaves 84-88). |
| Genre/form | dissertations. |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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|---|---|---|---|---|
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