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LEADER 02904cam a2200469Ia 4500

001 ocn810191476;

003 OCoLC;

005 20141212032708.0;

006 m o d ;

007 cr bn|||||||||;

008 120918s2012 ncua ob 000 0 eng d;

035 a| (Sirsi) o810191476;

035 a| (OCoLC)810191476;

049 a| EREE;

090 a| QR82.P78;

100 1 a| Williams, Melinda.?| UNAUTHORIZED;

245 10 a| Analysis of the hex regulon in Pseudomonas aeruginosa /c| by Melinda Williams.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2012.;

300 a| 46 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2012.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Thomas McConnell.;

500 a| Title from PDF t.p. (viewed Oct. 16, 2012).;

520 3 a| Pseudomonas aeruginosa is an opportunistic pathogen whose defense mechanisms include biofilm production through carbohydrate metabolism. The activity of the enzymes in the hex regulon are required for catabolism of glucose and these activities are coordinately regulated. Previous work has shown that these enzymes are induced when grown on carbohydrates and catabolite repressed when grown on TCA cycle intermediates. The HexR protein is a negative transcriptional repressor and the Crc protein participates in regulation of these genes and has been shown to be necessary for biofilm formation. The main objective of this study was to analyze control of these genes in different growth mediums and determine if Crc is a transcriptional protein or a post-transcriptional controller of the regulon. The transcriptional start sites for zwf and glk were mapped by primer extension assays. Comparisons of glk between wild type and a hexR mutant revealed the possibility of transcription without HexR control. Analysis of zwf mRNA showed that Crc affects mRNA stability and is acting at a post-transcriptional level.;

504 a| Includes bibliographical references.;

650 0 a| Pseudomonas aeruginosax| Molecular aspects.=| ^A717;

653 a| Molecular biology;

700 1 a| McConnell, Thomas J.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3960;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3108531;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3108531-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/18/2012x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3108531-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/18/2012x| ETDz| HERESOURCE;

Analysis of the hex regulon in Pseudomonas aeruginosa / by Melinda Williams.

Author/creator	Williams, Melinda
Other author	McConnell, Thomas J.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2012.
Description	46 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Pseudomonas aeruginosa--Molecular aspects.

Summary	Pseudomonas aeruginosa is an opportunistic pathogen whose defense mechanisms include biofilm production through carbohydrate metabolism. The activity of the enzymes in the hex regulon are required for catabolism of glucose and these activities are coordinately regulated. Previous work has shown that these enzymes are induced when grown on carbohydrates and catabolite repressed when grown on TCA cycle intermediates. The HexR protein is a negative transcriptional repressor and the Crc protein participates in regulation of these genes and has been shown to be necessary for biofilm formation. The main objective of this study was to analyze control of these genes in different growth mediums and determine if Crc is a transcriptional protein or a post-transcriptional controller of the regulon. The transcriptional start sites for zwf and glk were mapped by primer extension assays. Comparisons of glk between wild type and a hexR mutant revealed the possibility of transcription without HexR control. Analysis of zwf mRNA showed that Crc affects mRNA stability and is acting at a post-transcriptional level.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Thomas McConnell.
General note	Title from PDF t.p. (viewed Oct. 16, 2012).
Dissertation note	M.S. East Carolina University 2012.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Analysis of the hex regulon in Pseudomonas aeruginosa / by Melinda Williams.

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