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001 ocn810191473;

003 OCoLC;

005 20141212032707.0;

006 m o d ;

007 cr bn|||||||||;

008 120918s2012 ncua ob 000 0 eng d;

035 a| (Sirsi) o810191473;

035 a| (OCoLC)810191473;

049 a| EREE;

090 a| QH450;

100 1 a| Vick, Stephen.?| UNAUTHORIZED;

245 10 a| Validation of candidate genes in response to versican manipulation in developing synovial joints /c| by Stephen Ryan Vick.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2012.;

300 a| 66 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2012.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Anthony Capehart.;

500 a| Title from PDF t.p. (viewed Oct. 16, 2012).;

520 3 a| Little is understood about the complex process of synovial joint formation in early limb development. It has been shown that versican is highly expressed in the extracellular matrix of these joints (Snow et al., 2005; Shepard et al., 2007) and the knockdown of versican leads to malformation of the interzone, tissues that form the articular cartilage and synovial cavity (Nagchowdhuri et al., 2012). It is also thought that these effects impact gene expression in cells that are involved in the developing joint. Versican is chondroitin sulfate proteoglycan. It has four functional-modules: the N-terminal domain, the C-terminal domain, and GAG-[alpha], and GAG-[beta] chondroitin sulfate attachment regions. The N-terminal domain is also known as the G1 domain and the C-terminal domain is also known as the G3 domain (Kimata et al., 1986 and Zimmerman et al., 1989). In previous studies versican protein expression has been reduced and the G1 domain has been over-expressed to observe how these changes in the developing joint tissue effect gene expression. This study is a validation of microarray data specifically focusing on hyaluronan and Wnt pathway genes as they pertain to the misexpression of versican. RT-PCR and real-time PCR were used in this study to validate expression of the genes chosen. Through the use of these techniques the degree of expression has been quantified and compared to the fold changes observed with the microarray data. Overall, G1 versican mediated gene expression in the developing joint with regard to hyaluronan and Wnt pathway transcript regulation were in agreement with results obtained by RNA array.;

504 a| Includes bibliographical references.;

650 0 a| Genetic regulation.=| ^A6251;

650 0 a| Joints.=| ^A964458;

650 0 a| Proteoglycans.=| ^A14614;

650 0 a| Chondroitin sulfates.=| ^A1133302;

653 a| Biology;

653 a| Molecular biology;

653 a| Developmental biology;

700 1 a| Capehart, Anthony A.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3958;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3108527;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3108527-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/18/2012x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3108527-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/18/2012x| ETDz| HERESOURCE;

Validation of candidate genes in response to versican manipulation in developing synovial joints / by Stephen Ryan Vick.

Author/creator	Vick, Stephen
Other author	Capehart, Anthony A.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2012.
Description	66 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Genetic regulation. Joints. Proteoglycans. Chondroitin sulfates.

Summary	Little is understood about the complex process of synovial joint formation in early limb development. It has been shown that versican is highly expressed in the extracellular matrix of these joints (Snow et al., 2005; Shepard et al., 2007) and the knockdown of versican leads to malformation of the interzone, tissues that form the articular cartilage and synovial cavity (Nagchowdhuri et al., 2012). It is also thought that these effects impact gene expression in cells that are involved in the developing joint. Versican is chondroitin sulfate proteoglycan. It has four functional-modules: the N-terminal domain, the C-terminal domain, and GAG-[alpha], and GAG-[beta] chondroitin sulfate attachment regions. The N-terminal domain is also known as the G1 domain and the C-terminal domain is also known as the G3 domain (Kimata et al., 1986 and Zimmerman et al., 1989). In previous studies versican protein expression has been reduced and the G1 domain has been over-expressed to observe how these changes in the developing joint tissue effect gene expression. This study is a validation of microarray data specifically focusing on hyaluronan and Wnt pathway genes as they pertain to the misexpression of versican. RT-PCR and real-time PCR were used in this study to validate expression of the genes chosen. Through the use of these techniques the degree of expression has been quantified and compared to the fold changes observed with the microarray data. Overall, G1 versican mediated gene expression in the developing joint with regard to hyaluronan and Wnt pathway transcript regulation were in agreement with results obtained by RNA array.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Anthony Capehart.
General note	Title from PDF t.p. (viewed Oct. 16, 2012).
Dissertation note	M.S. East Carolina University 2012.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Validation of candidate genes in response to versican manipulation in developing synovial joints / by Stephen Ryan Vick.

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