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001 ocn794383495;

003 OCoLC;

005 20141212030016.0;

006 m d ;

007 cr bn|||||||||;

008 120529s2012 ncua ob 000 0 eng d;

035 a| (Sirsi) o794383495;

035 a| (OCoLC)794383495;

040 a| EREc| EREd| EREd| UtOrBLW;

049 a| EREE;

090 a| QK625.M7;

100 1 a| Worley, Charles.?| UNAUTHORIZED;

245 10 a| Lateral gene transfer between bacteria and Trichoderma reesei /c| by Charles Worley.;

260 a| Greenville, N.C.] :b| East Carolina University,c| 2012.;

300 a| 64 pages :b| illustrations (color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2012.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Jinling Huang.;

500 a| Title from PDF t.p. (viewed July 26, 2012).;

520 3 a| Lateral gene transfer (LGT) has played an important role in shaping the `tree of life'. It has promoted evolution by generating gene redundancies, which allow for more freedom in terms of allowed mutations in a genome, and by introducing new metabolic abilities to organisms. An example, with practical implications, is the cellulase activity of the fungus Trichoderma reesei which degrades cellulose in biofuel production, environmental waste cleanup, and paper processing. While partial optimization of conditions for T. reesei to express enzymes might be achieved through controlling experimental conditions, potentially the most effective approach would be to bring the power of genetic engineering to bear. If the digestive enzymes in T. reesei are the result of LGT, inserting bacterial copies of these genes or their regulators into the T. reesei genome may increase enzymatic activity. Furthermore, an appropriate examination of the entire genome for LGT could lead to insights into the evolution of T. reesei. This study performed phylogenomic analyses to identify LGT in the genome of T. reesei, specifically those genes transferred from bacteria. Three computational programs were used to identify LGT candidates. All identified LGT candidates were subject to further rigorous phylogenetic analyses to assess their evolutionary origins. We detected several cases of LGT between fungi and bacteria, only one of which was recently transferred to T. reesei. This leads us to conclude that LGT probably did not play a significant role in the recent evolution of T. reesei.;

504 a| Includes bibliographical references.;

650 0 a| Trichoderma reeseix| Genetics.=| ^A1056834;

650 0 a| Bacterial genetics.=| ^A5320;

650 0 a| Genetic transformation.=| ^A137175;

653 a| Biology;

653 a| Bioinformatics;

700 1 a| Huang, Jinling.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3827;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 3063833;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3063833-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 5/29/2012x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 3063833-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 5/29/2012x| ETDz| HERESOURCE;

Lateral gene transfer between bacteria and Trichoderma reesei / by Charles Worley.

Author/creator	Worley, Charles
Other author	Huang, Jinling.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	Greenville, N.C.] : East Carolina University, 2012.
Description	64 pages : illustrations (color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Trichoderma reesei--Genetics. Bacterial genetics. Genetic transformation.

Summary	Lateral gene transfer (LGT) has played an important role in shaping the `tree of life'. It has promoted evolution by generating gene redundancies, which allow for more freedom in terms of allowed mutations in a genome, and by introducing new metabolic abilities to organisms. An example, with practical implications, is the cellulase activity of the fungus Trichoderma reesei which degrades cellulose in biofuel production, environmental waste cleanup, and paper processing. While partial optimization of conditions for T. reesei to express enzymes might be achieved through controlling experimental conditions, potentially the most effective approach would be to bring the power of genetic engineering to bear. If the digestive enzymes in T. reesei are the result of LGT, inserting bacterial copies of these genes or their regulators into the T. reesei genome may increase enzymatic activity. Furthermore, an appropriate examination of the entire genome for LGT could lead to insights into the evolution of T. reesei. This study performed phylogenomic analyses to identify LGT in the genome of T. reesei, specifically those genes transferred from bacteria. Three computational programs were used to identify LGT candidates. All identified LGT candidates were subject to further rigorous phylogenetic analyses to assess their evolutionary origins. We detected several cases of LGT between fungi and bacteria, only one of which was recently transferred to T. reesei. This leads us to conclude that LGT probably did not play a significant role in the recent evolution of T. reesei.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Jinling Huang.
General note	Title from PDF t.p. (viewed July 26, 2012).
Dissertation note	M.S. East Carolina University 2012.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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