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001 ocn701552792;

003 OCoLC;

005 20141212055102.0;

006 m d ;

007 cr bn|||||||||;

008 110211s2010 ncua ob 000 0 eng d;

035 a| (Sirsi) o701552792;

035 a| (OCoLC)701552792;

040 a| EREc| EREd| EREd| UtOrBLW;

049 a| EREE;

090 a| QC475.5;

100 1 a| Hollis, Sarah Elizabeth.?| UNAUTHORIZED;

245 10 a| Exploring the bystander effect following very low dose radiation /c| by Sarah Elizabeth Hollis.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2010.;

300 a| 70 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2010.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Roberta Johnke.;

500 a| Title from PDF t.p. (viewed April 4, 2011).;

520 3 a| Bystander effects are defined as the phenomenon in which unirradiated cells respond biologically when their neighbors are irradiated. The exact mechanisms of these cellular responses are still not known, especially in the region considered to be very low dose radiation (<20cGy). Many studies have shown cytokine levels are altered following exposure to radiation and may play a significant role in bringing about bystander responses. A non-tumorigenic (MCF10A) and tumorigenic (A375) cell line were used to determine if chemical factors released from irradiated MCF10A cells will bring about bystander-induced proliferation on naïve (unirradiated) A375 or MCF10A cells using the Irradiated Conditioned Medium (ICM) model. A transfer of conditioned medium 5 hours post-irradiation appeared to bring about a marked bystander response in the naïve A375 cultures which were observed to be maximal between 2 and 6 cGy. This response was not observed at the 1 hour transfer of conditioned medium. Additionally, the data suggest that there is no temporal correlation between the proliferative bystander effects elicited and the levels of IL-6 within the conditioned medium. The data indicate that the MCF10A ICM IL-8 levels were statistically significant only at the 6 cGy dose at 1 hour. Although a significant proliferative bystander effect was observed in A375 cells when exposed to 5 hour MCF10A ICM, the data suggest that both IL-6 and IL-8 do not contribute significantly to this proliferative response.;

504 a| Includes bibliographical references.;

650 0 a| Low-level radiationx| Dose-response relationshipx| Research.=| ^A860044;

650 0 a| Cytokines.=| ^A249807;

650 0 a| Biological response modifiers.=| ^A650340;

653 a| Molecular Biology;

700 1 a| Johnke, Roberta M.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3147;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 2399309;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2399309-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 2/14/2011x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2399309-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 2/14/2011x| ETDz| HERESOURCE;

Exploring the bystander effect following very low dose radiation / by Sarah Elizabeth Hollis.

Author/creator	Hollis, Sarah Elizabeth
Other author	Johnke, Roberta M.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2010.
Description	70 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Low-level radiation--Dose-response relationship. --Research. Cytokines. Biological response modifiers.

Summary	Bystander effects are defined as the phenomenon in which unirradiated cells respond biologically when their neighbors are irradiated. The exact mechanisms of these cellular responses are still not known, especially in the region considered to be very low dose radiation (<20cGy). Many studies have shown cytokine levels are altered following exposure to radiation and may play a significant role in bringing about bystander responses. A non-tumorigenic (MCF10A) and tumorigenic (A375) cell line were used to determine if chemical factors released from irradiated MCF10A cells will bring about bystander-induced proliferation on naïve (unirradiated) A375 or MCF10A cells using the Irradiated Conditioned Medium (ICM) model. A transfer of conditioned medium 5 hours post-irradiation appeared to bring about a marked bystander response in the naïve A375 cultures which were observed to be maximal between 2 and 6 cGy. This response was not observed at the 1 hour transfer of conditioned medium. Additionally, the data suggest that there is no temporal correlation between the proliferative bystander effects elicited and the levels of IL-6 within the conditioned medium. The data indicate that the MCF10A ICM IL-8 levels were statistically significant only at the 6 cGy dose at 1 hour. Although a significant proliferative bystander effect was observed in A375 cells when exposed to 5 hour MCF10A ICM, the data suggest that both IL-6 and IL-8 do not contribute significantly to this proliferative response.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Roberta Johnke.
General note	Title from PDF t.p. (viewed April 4, 2011).
Dissertation note	M.S. East Carolina University 2010.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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