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003 OCoLC;

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007 cr bn|||||||||;

008 110211s2010 ncua ob 000 0 eng d;

035 a| (Sirsi) o701552784;

035 a| (OCoLC)701552784;

040 a| EREc| EREd| EREd| UtOrBLW;

049 a| EREE;

090 a| QP572.P7;

100 1 a| Daly, Seán.=| ^A1038136;

245 10 a| Localization and changes of nuclear progesterone receptors in zebrafish oocytes and adjacent follicular cells /c| by Sean Daly.;

260 a| [Greenville, N.C.] :b| East Carolina University,c| 2010.;

300 a| 86 pages :b| illustrations (some color), digital, PDF file;

336 a| text2| rdacontent;

337 a| computer2| rdamedia;

338 a| online resource2| rdacarrier;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2010.;

500 a| Presented to the faculty of the Department of Biology.;

500 a| Advisor: Yong Zhu.;

500 a| Title from PDF t.p. (viewed March 23, 2011).;

520 3 a| The nuclear progesterone receptor (Pgr) is one of the major mediators for progestin signaling during oocyte ovulation in vertebrates. However, any roles Pgr may play in oocyte growth, and especially in the final oocyte maturation, are controversial. Due to the small size of the oocytes and difficulty in separation of encircled follicular cells from oocytes, little information is available on the location and changes of Pgr in vertebrates. We study the roles of the Pgr in the ovaries of zebrafish, which release eggs daily, by localizing and comparing the changes of Pgr in the oocytes and enclosed follicular layers at all developmental stages and from different times of the day or after treatment with various hormones. The pgr transcript and Pgr protein were expressed in oocytes and follicular cells of early developmental stages (stage I and II). In contrast, Pgr was expressed abundantly in the follicular cells surrounding late developmental stage oocytes (stage IV) but completely absent from stage IV oocytes. Furthermore, the most significant daily changes of pgr transcript were observed in the stage IV follicular cells with the highest level observed at 6 am prior to ovulation and the lowest level at 9 pm. The levels of pgr transcript were upregulated by 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (DHP) in both stages of I-II and IV oocytes. Intriguingly, estrogen (E2) and human chorionic gonadotropins (hCG) significantly suppressed pgr expression in stage I-II oocytes, while enhancing pgr expression in stage IV oocytes. Presence of Pgr in both oocytes and follicular cells of early stages (stages I-II) suggest roles of Pgr in oocyte growth. Abundant expression and dramatic changes of Pgr in the follicular cells of late stage (stage IV) indicate roles of Pgr during oocyte ovulation. Absence and lack of changes of Pgr in the stage IV ooctyes do not support the role of Pgr in final oocyte maturation.;

504 a| Includes bibliographical references.;

650 0 a| Progesteronex| Receptors.=| ^A162263;

650 0 a| Zebra daniox| Eggs.=| ^A584582;

650 0 a| Nuclear receptors (Biochemistry)=| ^A828141;

653 a| Molecular Biology;

700 1 a| Zhu, Yong.=| ^A1041908;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/3145;

949 o| jgml;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 2399299;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2399299-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 2/14/2011x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 2399299-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 2/14/2011x| ETDz| HERESOURCE;

Localization and changes of nuclear progesterone receptors in zebrafish oocytes and adjacent follicular cells / by Sean Daly.

Author/creator	Daly, Seán
Other author	Zhu, Yong.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication Info	[Greenville, N.C.] : East Carolina University, 2010.
Description	86 pages : illustrations (some color), digital, PDF file
Supplemental Content	Access via ScholarShip
Subjects	Progesterone--Receptors. Zebra danio--Eggs. Nuclear receptors (Biochemistry)

Summary	The nuclear progesterone receptor (Pgr) is one of the major mediators for progestin signaling during oocyte ovulation in vertebrates. However, any roles Pgr may play in oocyte growth, and especially in the final oocyte maturation, are controversial. Due to the small size of the oocytes and difficulty in separation of encircled follicular cells from oocytes, little information is available on the location and changes of Pgr in vertebrates. We study the roles of the Pgr in the ovaries of zebrafish, which release eggs daily, by localizing and comparing the changes of Pgr in the oocytes and enclosed follicular layers at all developmental stages and from different times of the day or after treatment with various hormones. The pgr transcript and Pgr protein were expressed in oocytes and follicular cells of early developmental stages (stage I and II). In contrast, Pgr was expressed abundantly in the follicular cells surrounding late developmental stage oocytes (stage IV) but completely absent from stage IV oocytes. Furthermore, the most significant daily changes of pgr transcript were observed in the stage IV follicular cells with the highest level observed at 6 am prior to ovulation and the lowest level at 9 pm. The levels of pgr transcript were upregulated by 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (DHP) in both stages of I-II and IV oocytes. Intriguingly, estrogen (E2) and human chorionic gonadotropins (hCG) significantly suppressed pgr expression in stage I-II oocytes, while enhancing pgr expression in stage IV oocytes. Presence of Pgr in both oocytes and follicular cells of early stages (stages I-II) suggest roles of Pgr in oocyte growth. Abundant expression and dramatic changes of Pgr in the follicular cells of late stage (stage IV) indicate roles of Pgr during oocyte ovulation. Absence and lack of changes of Pgr in the stage IV ooctyes do not support the role of Pgr in final oocyte maturation.
General note	Presented to the faculty of the Department of Biology.
General note	Advisor: Yong Zhu.
General note	Title from PDF t.p. (viewed March 23, 2011).
Dissertation note	M.S. East Carolina University 2010.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Localization and changes of nuclear progesterone receptors in zebrafish oocytes and adjacent follicular cells / by Sean Daly.

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