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LEADER 03552ctm a2200517 i 4500

001 ocm09507886;

003 OCoLC;

005 20251021154320.0;

008 830513s1982 xx a bm 000 0 eng d;

035 a| (Sirsi) o09507886;

035 a| (OCoLC)9507886;

049 a| EREE;

050 4 a| QP363b| .K8x 1982;

100 1 a| Kurtz, Catherine L.,e| author.?| UNAUTHORIZED;

245 10 a| Studies on the preparation and maintenance of primary neural tissue cultures from the crayfish, Procambarus clarkii /c| by Catherine L. Kurtz.;

264 0 c| 1982.;

300 a| 97 pages :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1982;

500 a| "Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology.";

500 a| Advisor: Gerhard W. Kalmus;

520 3 a| To establish and evaluate growth of ganglion and axon explants of the crayfish, Procambarus clarkii, explants of both cell types were cultured on coverslips for 3-20 days in 18 media that were adjusted to crayfish tissue fluid osmolality. To insure the cultures were free of bacterial and mycotic contamination, antimicrobial agents were utilized in concentrations necessary to restrict growth of these microorganisms. All culture media were supplemented with selected growth enhancers of which insulin, transferrin, and in some cases, nerve growth factor were most successful for maintaining viable explants and supporting growth. Pretreatment of tissues with collagenase enhanced explant affinity for the substratum. Cultures were incubated at temperatures ranging from 15° to 37°C in an air or 95% 02-5% CO2 atmosphere; optimum conditions were 25° and 37°C in an atmosphere of 95% 02-5% CO2. Tissue maintenance and growth was evaluated by light microscopy, and crayfish nerve tissue was observed using light and electron microscopy.;

504 a| Includes bibliographical references (leaves 89-94).;

650 0 a| Tissue culture.=| ^A3430;

650 0 a| Nerve tissuex| Cultures and culture media.=| ^A146219;

650 7 a| Nerve tissuex| Cultures and culture media.2| fast0| (OCoLC)fst01036053;

650 7 a| Tissue culture.2| fast0| (OCoLC)fst01151481;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

700 1 a| Kalmus, Gerhard W.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/11226;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

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596 a| 1 4;

998 a| 201047;

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Studies on the preparation and maintenance of primary neural tissue cultures from the crayfish, Procambarus clarkii / by Catherine L. Kurtz.

Author/creator	Kurtz, Catherine L. author.
Other author	Kalmus, Gerhard W., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1982.
Description	97 pages : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Tissue culture. Nerve tissue--Cultures and culture media.

Summary	To establish and evaluate growth of ganglion and axon explants of the crayfish, Procambarus clarkii, explants of both cell types were cultured on coverslips for 3-20 days in 18 media that were adjusted to crayfish tissue fluid osmolality. To insure the cultures were free of bacterial and mycotic contamination, antimicrobial agents were utilized in concentrations necessary to restrict growth of these microorganisms. All culture media were supplemented with selected growth enhancers of which insulin, transferrin, and in some cases, nerve growth factor were most successful for maintaining viable explants and supporting growth. Pretreatment of tissues with collagenase enhanced explant affinity for the substratum. Cultures were incubated at temperatures ranging from 15° to 37°C in an air or 95% 02-5% CO2 atmosphere; optimum conditions were 25° and 37°C in an atmosphere of 95% 02-5% CO2. Tissue maintenance and growth was evaluated by light microscopy, and crayfish nerve tissue was observed using light and electron microscopy.
General note	"Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology."
General note	Advisor: Gerhard W. Kalmus
Dissertation note	M.S. East Carolina University 1982
Bibliography note	Includes bibliographical references (leaves 89-94).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Studies on the preparation and maintenance of primary neural tissue cultures from the crayfish, Procambarus clarkii / by Catherine L. Kurtz.

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