The frequency and inducibility of staphylococcus epidermidis bacteriophages and their potential for use in a typing series / by James Edward Bostian.

Author/creator Bostian, James Edward author.
Other author Allen, Wendall E., degree supervisor.
Other author East Carolina University. Department of Biology.
Format Theses and dissertations
Production1976.
Description51 leaves : illustrations ; 28 cm
Supplemental ContentAccess via ScholarShip
Subjects

Summary Until recently Staphylococcus epidermidis has been considered by clinical laboratories as nonpathogenic and regarded as a contaminant in bacterial isolates from infections, S. epidermidis has been increasingly associated with certain diseases such as endocarditis (Holt, 1969), urinary tract infections (Mabeck, 1969), cardiac surgery complications (Blouse et al., 1975), and osteomyelitis (Overturf and Balfour, 1975). Baird-Parker Biotype 1 cultures of S. epidermidis have been most often found by Holt (1969) and other workers implicated in infections. Due to the rising significance of S. epidermidis in disease, a phage-typing system similar to the one already used in epidemiological studies of Staphylococcus aureus seems desirable. Lysogeny in S. epidermidis has been found to be as frequent as in S. aureus (Pulverer et al, 1974). Mitomycin C and UV-irradiation have been the most used methods of induction of staphylococci. Verhoef et al. (1972), Dean et al. (1973) and Pulverer et al. (1975) used these methods to obtain phages useful for typing S. epidermidis cultures predominately of European origin. To facilitate development of a phage-typing system for S. epidermidis, we have identified a group of for S. epidermidis cultures particularly sensitive to phages useful in screening induction products. This paper describes the frequency of lysogeny in 241 strains of S. epidermidis from the U. S. A., compares the effectiveness of Mitomycin C and UV-irradiation as inducing agents, and presents a group 2 of S. epidemies phages that may be of value in phage typing S. epidermidis cultures.
Local noteJoyner-"Presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology."
General notePresented to the Faculty of the Department of Biology
General noteAdvisor: Wendall E. Allen
Dissertation noteM.S. East Carolina University
Bibliography noteIncludes bibliographical references (leaves 47-51).
Genre/formAcademic theses.
Genre/formAcademic theses.
Genre/formThèses et écrits académiques.

Availability

Library Location Call Number Status Item Actions
Joyner University Archives ASK AT SPECIAL COLLECTIONS DESK ✔ Available Request Material
Electronic Resources Access Content Online ✔ Available