Ubiquitin-proteasome protocols / edited by Cam Patterson, Douglas M. Cyr.

Other author Patterson, Cam.
Other author Cyr, Douglas M.
Format Book
Publication InfoTotowa, N.J. : Humana Press, ©2005.
Descriptionxi, 381 pages : illustrations ; 24 cm.
Subjects
Ubiquitin--metabolism.
Cysteine Endopeptidases.
Multienzyme Complexes.

SeriesMethods in molecular biology ; 301
Methods in molecular biology (Clifton, N.J.) v. 301. ^A682607
Contents Small-molecule inhibitors of proteasome activity / Maria Gaczynska and Pawel A. Osmulski -- Purification of E1 and E1-like enzymes / Arthur L. Haas -- Assays for RING family ubiquitin ligases / Manabu Furukawa, Paul S. Andrews, and Yue Xiong -- Ubiquitin chain synthesis / Shahri Raasi and Cecile M. Pickart -- Purification of proteasomes, proteasome subcomplexes, and proteasome-associated proteins from budding yeast / David S. Leggett, Michael H. Glickman, and Daniel Finley -- Recognition and processing of misfolded proteins by PA700, the 19S regulatory complex of the 26S proteasome / Chang-Wei Liu ... [et al.] -- Cell-free assay for ubiquitin-independent proteasomal protein degradation / Chaim Kahana and Yuval Reiss --
Contents Assays of proteasome-dependent cleavage products / Stefan Tenzer and Hansjörg Schild -- Identification of components of protein complexes / Carol E. Parker ... [et al.] -- Mass spectrometric determination of protein ubiqitination / Carol E. Parker ... [et al.] -- Reconstitution of endoplasmic reticulum-associated degradation using yeast membranes and cytosol / Robert J. Lee, Ardythe A. McCracken, and Jeffrey L. Brodsky -- Reticulocyte lysate as a model system to study endoplasmic reticulum membrane protein degradation / Eric Carlson ... [et al.] -- Deubiquitinating enzyme purifucation, assay inhibitors, and characterization / Nathaniel S. Russell and Keith D. Wilkinson -- Measuring ubiquitin conjugation in cells / Edward G. Mimnaugh and Leonard M. Neckers -- Assays for proteasome assembly and maturation / R. Jürgen Dohmen ... [et al.].
Contents N-terminal ubiquitination / Aaron Ciechanover -- Quantitating defective ribosome products / Shu-Bing Qian, Jack R. Bennink, and Jonathan W. Yewdell --endoplasmic reticulum-associated protein quality control and degradation: screen for ERAD mutants after ethylmethane sulfonate mutagenesis / Antje Schäfer and Dieter H. Wolf -- Endoplasmic reticulum-associated protein quality control and degradation: genome wide screen for ERAD components / Antje Schäfer and Dieter H. Wolf -- Cystic fibrosis transmembrane conductance regulator as a model substrate to study endoplasmic reticulum protein quality control in mammalian cells / J. Michael Younger ... [et al.] -- Aggresome formation / Michael J. Corboy, Philip J. Thomas, and W. Christian Wigley -- Detection of sumoylated proteins / Roland S. Hilgarth and Kevin D. Sarge -- Proteasome inhibitors in cancer therapy / Robert Z. Orlowski -- Parkinson's disease: assays for the ubiquitin ligase activity of neural parkin / Michael G. Schlossmacher and Hideki Shimura.
Bibliography noteIncludes bibliographical references and index.
LCCN 2004017480
ISBN1588292525 :

Availability

Library Location Call Number Status Item Actions
Laupus Books - Stacks QU 56 U151 2005 ✔ Available Place Hold