| Series |
Practical approach series ; 140
Practical approach series ; 140. ^A664597
|
| Contents |
Historical introduction / J.M. Gershoni -- Basic equipment and methods for protein blotting -- Introduction and basic set-up for protein transfer / Bonnie S. Dunbar -- Protein blotting and immunoblotting using nitrocellulose membranes / Antonio De Maio -- Protein blotting using polyvinylidene fluoride membranes / Michael A. Mansfield -- Protein blotting using semi-dry electrophoretic transfer equipment / Gunilla Jacobson. |
| Contents |
(cont) Principles and methods for preparing samples for protein transfer -- Transfer and blocking conditions in immunoblotting / A. Van Dam -- Sample preparation for protein electrophoresis and transfer / Vaughan H. Lee, Bonnie S. Dunbar -- Strategy for purification of organelles from parasites: protein blotting as one of the initial steps / David W. Sammons -- Detection methods and uses in immunoblotting -- Radiometric methods for detection in blots / R.A. Laskey. |
| Contents |
(cont) Bioluminescence-enhanced detection systems in protein blotting / Reinhard Erich Geiger -- Chemiluminescent detection systems for protein blotting / Ian Durrant, Sue Fowler -- Applications of protein blotting -- Detection and characterization of glycoprotein carbohydrate chains after electrophoretic separation / Tatsuro Irimura, Hiroto Kawashima -- DNA binding of immobilized proteins / Constantin N. Flytzanis. |
| Contents |
(cont) Use of peptide ligands for the detection of binding proteins by western blotting / Paul Hossenlopp, Michel Binoux -- Use of blotted proteins as immunogens / Boguslaw Szewczyk, David R. Harper -- Amino acid sequence analysis of blotted proteins / Richard G. Cook -- Renaturative catalytic blotting of enzyme proteins / Jia-Shi Zhu, Gary M. Gray. |
| Bibliography note | Includes bibliographical references and index. |
| LCCN | 93042043 |
| ISBN | 0199634386 (hbk.) : |
| ISBN | 0199634378 (pbk.) : |
