Fatty acid transporters in adipose tissue : effect of aerobic running phenotype and high fat feeding / by Esther Otelia Victoria Granville.
| Author/creator | Granville, Esther Otelia Victoria author. |
| Other author | Lust, Robert M., degree supervisor. |
| Other author | East Carolina University. Department of Exercise and Sport Science. |
| Format | Theses and dissertations |
| Production | 2008. |
| Description | x, 73 leaves : illustrations (some color) ; 29 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Studies show that a breakdown of normal metabolic functioning is a relevant process in the development of metabolic disease. A novel rat model, phenotypically selected for inherent endurance running capacity, has been developed that allows further investigation into the differences in substrate handling based on intrinsic aerobic capacity, but without the confounding effects of active exercise training-induced gene expression. When the rats were maintained on a high fat diet, low capacity running (LCR) animals demonstrated significant weight gain, including a significant increase in adipose tissue mass, while the high capacity running (HCR) animals did not, despite similar energy intake patterns. The aim of the current study was to determine whether differences in the level of the fatty acid transporters FATP4 and CD36 in adipose tissue between ECR and HCR rats on chow or high fat diet (HFD) could be correlated with differences in weight gain observed in these groups. No significant differences in FATP4 content were seen between groups on chow or HFD. Under basal conditions, there were no differences between the HCR and LCR rats in the level of CD36 (208758 ± 50881 AU vs. 265627 ± 95290 AU). Rats that were subjected to the 4 week HFD showed increased CD36 content when compared to their chow counterparts, but these results were found to be significant in the HFD HCR rats only (444348 ± 140649, p < 0.005). These results indicate that the increase in weight gain and adiposity observed in the LCR rats on HFD compared to HCR rats on HFD likely was not the result of increased content of the fatty acid transporters FATP4 and CD36, and HCR rats were resistant to adiposity despite an increase in adipose fatty acid transport protein. These data suggest that there is a strong genetic basis for animals with increased aerobic capacity to withstand the stress of dietary fat, but the mechanisms responsible for it remain unclear. |
| General note | Presented to the faculty of the Department of Exercise and Sport Science. |
| General note | Advisor: Robert M. Lust |
| Dissertation note | M.S. East Carolina University 2008 |
| Bibliography note | Includes bibliographical references (leaves 41-47). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |