A study of the Salmonella flagellar antigens that are bound by a murine myeloma protein, MOPC 467 / by Jeffrey Scott Miller.
| Author/creator | Miller, Jeffrey Scott author. |
| Other author | Smith, A. Mason, degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Publication Info | Greenville, N.C. : East Carolina University, 1978. |
| Description | 69 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | It has been previously reported that a murine IgA myeloma protein, UOPC 467. from BALB/c mice binds flagellar protein (flagellin) from several serotypically distinct species of Salmonella, in this study, affinity chromatography has been used to purify cyanogen bromide digest polypeptide from; flagellin which reacts with purified MOPC- 467 myeloma protein (M467). M467 purified by immunoadsorption on a flagellin-CH- Sepharose 4B column was coupled to CNBr activated Sepharose 4B. CNBr digests of purified flagellin from various species of Salmonella were passed over the M467-S4B columns and fractions reacting with M467 were eluted in an acid buffer. The eluted fractions were further chromatographed on a G-50 Sephadex column. Various species of Salmonella flagellin, CNBr digests of flagellin, and G-50 fractions of affinity elutions were analyzed by polyacrylamide gel electrophoresis in Sodium Dodecyl Sulphate (SDS) including gradient gels and double diffusion in agar gel (Ouchterlony). An 18,000 MW polypeptide was found which was common in all the species studied suggesting an antigenic determinant common to all salmonella flagella. Passive hemagglutination inhibition was used to detect quantitative differences in binding capacities of flagellin from seven species of Salmonella. It was found that the homogeneous myeloma protein M467 bound with varying avidity flagellin from the different species. |
| General note | "A thesis presented to the faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology." |
| General note | Advisor: A. Mason Smith |
| Dissertation note | M.S. East Carolina University 1978 |
| Bibliography note | Includes bibliographical references (leaves 66-69). |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | ✔ Available |