Tools

LEADER 04442cam a2200481 i 4500

001 ocm03474949;

003 OCoLC;

005 20240719122615.0;

008 771208s1977 ncua b s000 0 eng d;

035 a| (Sirsi) o03474949;

035 a| (OCoLC)3474949;

049 a| EREE;

050 4 a| QP188.P26b| F7x;

100 1 a| Frost, Cecil C.,e| author.=| ^A98859;

245 10 a| Studies on the endocrine cell types of the fetal rat pancreas and gastrointestinal tract /c| by Cecil C. Frost.;

264 0 c| 1977.;

300 a| 165 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1977;

500 a| "A thesis presented to the Faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology.";

500 a| Advisor: Michael R. Schweisthal;

520 3 a| This thesis presents three histochemical studies on the fetal rat Islets of Langerhans, and notes on staining of fetal gastrointestinal endocrine cells. In the first section, staining techniques were developed for differentiating fetal A, B and D cells, which were the only well documented cell types of the pancreatic islets when these studies were begun. Two dependable methods for identifying B cells had existed for some time (aldehyde fuchsin and the fluorescent antibody method for insulin), but no stains had been successful for fetal A or D cells. In the process of developing these techniques, a 4th cell type was found. The second section is a developmental study. The stains derived above were used to determine the earliest appearance of each of the four cell types in the fetal pancreas. Then the development of the islets of Langerhans was studied, noting changes in size, shape and relative abundance of the four cell types from the earliest stainable stage, each day through birth and the first postnatal week, and then at weekly intervals thereafter until adulthood. The most remarkable finding was the abundance of the 4th cell type in the fetus, its decline after birth, and scarcity in the adult. In the third section, the same techniques were used to follow changes in islet morphology and islet cell populations which occurred in pancreas grown in organ culture. Thirteen day old fetal pancreas was placed in culture on sterile grids and collected for study after 10 days. Eighteen day old pancreas was also placed in culture and collected every other day for periods up to 30 days. While all four cell types were still present at the end of 30 days, drastic changes were seen in the relative proportions of the cell types. A mechanism was also observed by which apparently healthy islets were being destroyed by fusion with fluid-filled exocrine sacs, which may have contained proteolytic enzymes. Ways to overcome this problem were suggested. Virtually no work has been done on the histochemistry of fetal gastrointestinal endocrine cells. The fourth section contains a review of the literature, and some notes on endocrine cells of the fetal gastrointestinal tract, seen when this material was included on slides of fetal pancreas being stained for the studies above.;

504 a| Includes bibliographical references (leaves 153-165).;

650 0 a| Pancreas.=| ^A7251;

650 0 a| Endocrinology.=| ^A554;

650 0 a| Gastroenterology.=| ^A1175;

650 7 a| Endocrinology.2| fast0| (OCoLC)fst00909749;

650 7 a| Gastroenterology.2| fast0| (OCoLC)fst00938842;

650 7 a| Pancreas.2| fast0| (OCoLC)fst01051970;

700 1 a| Schweisthal, Michael R.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/11145;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 128174;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 188736d| 9/21/2000l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 9/21/2000x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 128174-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 7/19/2024x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 128174-4001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 7/19/2024x| ETDz| HERESOURCE;

Studies on the endocrine cell types of the fetal rat pancreas and gastrointestinal tract / by Cecil C. Frost.

Author/creator	Frost, Cecil C. author.
Other author	Schweisthal, Michael R., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1977.
Description	165 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Pancreas. Endocrinology. Gastroenterology.

Summary	This thesis presents three histochemical studies on the fetal rat Islets of Langerhans, and notes on staining of fetal gastrointestinal endocrine cells. In the first section, staining techniques were developed for differentiating fetal A, B and D cells, which were the only well documented cell types of the pancreatic islets when these studies were begun. Two dependable methods for identifying B cells had existed for some time (aldehyde fuchsin and the fluorescent antibody method for insulin), but no stains had been successful for fetal A or D cells. In the process of developing these techniques, a 4th cell type was found. The second section is a developmental study. The stains derived above were used to determine the earliest appearance of each of the four cell types in the fetal pancreas. Then the development of the islets of Langerhans was studied, noting changes in size, shape and relative abundance of the four cell types from the earliest stainable stage, each day through birth and the first postnatal week, and then at weekly intervals thereafter until adulthood. The most remarkable finding was the abundance of the 4th cell type in the fetus, its decline after birth, and scarcity in the adult. In the third section, the same techniques were used to follow changes in islet morphology and islet cell populations which occurred in pancreas grown in organ culture. Thirteen day old fetal pancreas was placed in culture on sterile grids and collected for study after 10 days. Eighteen day old pancreas was also placed in culture and collected every other day for periods up to 30 days. While all four cell types were still present at the end of 30 days, drastic changes were seen in the relative proportions of the cell types. A mechanism was also observed by which apparently healthy islets were being destroyed by fusion with fluid-filled exocrine sacs, which may have contained proteolytic enzymes. Ways to overcome this problem were suggested. Virtually no work has been done on the histochemistry of fetal gastrointestinal endocrine cells. The fourth section contains a review of the literature, and some notes on endocrine cells of the fetal gastrointestinal tract, seen when this material was included on slides of fetal pancreas being stained for the studies above.
General note	"A thesis presented to the Faculty of the Department of Biology ... in partial fulfillment of the requirements for the degree Master of Science in Biology."
General note	Advisor: Michael R. Schweisthal
Dissertation note	M.S. East Carolina University 1977
Bibliography note	Includes bibliographical references (leaves 153-165).

Availability

Library	Location	Call Number	Status	Item Actions
Joyner	University Archives	ASK AT SPECIAL COLLECTIONS DESK	✔ Available	Request Material
	Electronic Resources	Access Content Online	✔ Available

Studies on the endocrine cell types of the fetal rat pancreas and gastrointestinal tract / by Cecil C. Frost.

Click here for more information about this title

Availability